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Covalent conjugation of bovine serum album and sugar beet pectin through Maillard reaction/laccase catalysis to improve the emulsifying properties
会议论文
13th International Hydrocolloids Conference (IHC), MAY 16-20, 2016
作者:
Chen, Hao
;
Ji, Aiguo
;
Qiu, Shuang
;
Liu, Yan
;
Zhu, Qiaomei
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/31
Sugar beet pectin
Bovine serum album
Maillard reaction
Laccase
Covalent conjugates
Capture and Dimethyl Labeling of Glycopeptides on Hydrazide Beads for Quantitative Glycoproteomics Analysis
期刊论文
analytical chemistry, 2012, 卷号: 84, 期号: 20, 页码: 8452-8456
作者:
Sun, Zhen
;
Qin, Hongqiang
;
Wang, Fangjun
;
Cheng, Kai
;
Dong, Mingming
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  |  
浏览/下载:17/0
  |  
提交时间:2013/10/11
Interaction of Human Serum Album and C-60 Aggregates in Solution
期刊论文
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 卷号: 12, 期号: 8, 页码: 4964—4974
Song, Maoyong
;
Liu, Shufang
;
Yin, Junfa
;
Wang, Hailin
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  |  
浏览/下载:21/0
  |  
提交时间:2012/10/16
Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles
期刊论文
JOURNAL OF CHROMATOGRAPHY A, 2010, 卷号: 1217, 期号: 28, 页码: 4663-4668
Qing, Lin-Sen
;
Xue, Ying
;
Zheng, Yi
;
Xiong, Jing
;
Liao, Xun
;
Ding, Li-Sheng
;
Li, Bo-Gang
;
Liu, Yi-Ming
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  |  
浏览/下载:10/0
  |  
提交时间:2011/07/08
Ligand fishing
Magnetic nanoparticles
Human serum album
Dioscorea nipponica
Saponins
Dioscin
Generation and characterization of a monoclonal antibody to penicillic acid from Penicillium cyclopium
期刊论文
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 卷号: 9, 期号: 21, 页码: 3026-3031
作者:
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浏览/下载:6/0
  |  
提交时间:2019/12/23
Penicillic acid is one of the main mycotoxins in moldy feedstuff and has toxic effect on livestock and poultry and probably humans due to food chain transmission. The objective of this study was to generate and characterize a monoclonal antibody to penicillic acid for the efficient detection of penicillic acid from Penicillium cyclopiumby immunological methods. To this end
penicillic acid was conjugated to bovine serum album (BSA) using the Mannich reaction and coupled with ovalbumin (OVA) by the method of 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC) to generate artificial antigens penicillic acid-BSA and penicillic acid-OVA. A hybridoma cell line was obtained after fusion of mouse SP2/0 myeloma cells with spleen cells of BALB/c mice immunized with artificial antigen penicillic acid-BSA. A monoclonal antibody specific against penicillic acid was produced in vivo by this hybridoma cell line. Further analysis revealed that the monoclonal antibody to penicillic acid was of the IgG1 subtype
with a titer of 1: 2.05 × 105. The antibody to penicillic acid had no or less cross-reaction with mycotoxins
including aflatoxin B1
zearalenone
T-2 toxin and fumonisins and more importantly
it assumed an affinity of about 1.54 × 108 liters per mol. Our ability to produce a monoclonal antibody to penicillic acid provides necessary groundwork for the effective detection of penicillic acid in various tissues of animals and human
using the immunocytochemistry
Western blots and enzyme-linked immunosorbent assay (ELISA).
Dynamic adsorption and characterization of phospholipid and mixed phospholipid/protein layers at liquid/liquid interfaces
期刊论文
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2008, 卷号: 140, 期号: 2, 页码: 67-76
作者:
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浏览/下载:15/0
  |  
提交时间:2019/04/09
Adsorption Dynamics
Lipid/protein Complex
Skin-like Film
Drop Profile Analysis Tensiometry
Liquid/liquid Interface
Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting
期刊论文
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2007, 卷号: 35, 期号: 1, 页码: 43-48
作者:
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浏览/下载:23/0
  |  
提交时间:2013/10/15
succinylated gelatin
modification degree
size exclusion chromatography
multi angle laser light scatting
trinitrobenzenesulfonic acid
determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting
期刊论文
chinesejournalofanalyticalchemistry, 2007, 卷号: 35, 期号: 1, 页码: 43
作者:
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  |  
浏览/下载:10/0
  |  
提交时间:2019/12/02
Tudy on drug displacement interactions by capillary electrophoresis-frontal analysis
期刊论文
Frontiers of Chemistry in China, 2006, 卷号: Vol.1 NO.2, 页码: 207-210
作者:
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浏览/下载:2/0
  |  
提交时间:2019/11/21
capillary electrophoresis-frontal analysis - human serum album in - displacement interaction
卟啉全内反射同步荧光法测定蛋白质
期刊论文
2005
陈莹
;
姚闽娜
;
唐尧基
;
李耀群
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浏览/下载:2/0
  |  
提交时间:2011/04/26
全内反射
同步荧光
卟啉
血清蛋白
Total internal reflection
Synchronous fluorescence
TPPS
Protein
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