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	Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting
	Tao, Liu; Zhang Gui-Feng; Zhou Wei-Bin; Su Zhi-Guo
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2007
卷号	35期号:1页码:43-48
关键词	succinylated gelatin modification degree size exclusion chromatography multi angle laser light scatting trinitrobenzenesulfonic acid
ISSN号	0253-3820
其他题名	Chin. J. Anal. Chem.
中文摘要	The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin.
英文摘要	The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	2,4,6-TRINITROBENZENESULFONIC ACID ; SCATTERING ; AMINES
收录类别	SCI
原文出处	://WOS:000246604700008
语种	英语
WOS记录号	WOS:000246604700008
公开日期	2013-10-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3445]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Tao, Liu,Zhang Gui-Feng,Zhou Wei-Bin,et al. Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(1):43-48.
APA	Tao, Liu,Zhang Gui-Feng,Zhou Wei-Bin,&Su Zhi-Guo.(2007).Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(1),43-48.
MLA	Tao, Liu,et al."Determination of modification degree of succinylated gelatin by size exclusion chromatography coupled with multi angle laser light scatting".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.1(2007):43-48.

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