determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting

	determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting
	Liu T(刘涛); Zhang GF(张贵锋); Zhou WB(周卫斌); Su ZG(苏志国)
刊名	chinesejournalofanalyticalchemistry
	2007
卷号	35 期号:1 页码:43
ISSN号	0253-3820
英文摘要	The clinical effectiveness of succinylated gelatin as a plasma substitute depends on its molar mass and free amino modification degree. In this study, a new method was developed to determine the modification degree of succinylated gelatin. Gelatin and succinylated gelatin were derived with trinitrobenzenesulfonic acid (TNBS). The molar masses of gelatin and succinylated gelatin were determined before and after TNBS-derivation by size exclusion chromatography coupled with multi angle laser light scatting (SEC-MALLS). The modification degree was obtained by determining the free amino groups of gelatins before and after succinylation. Human serum album in was used as a model protein to validate the method. The determined value was consistent with the calculated value. Actual samples were analyzed with the developed method. The result indicates that the mean modification degree of succinylated gelatins is 38%. This research indicates that size exclusion chromatography multi angle laser light scatting coupled with the trinitrobenzenesulfonic acid derivation is a possible method to determine the modification degree of succinylated gelatin.
语种	英语
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/35234]
专题	中国科学院过程工程研究所
作者单位	中国科学院过程工程研究所
推荐引用方式 GB/T 7714	Liu T,Zhang GF,Zhou WB,et al. determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting[J]. chinesejournalofanalyticalchemistry,2007,35(1):43.
APA	刘涛,张贵锋,周卫斌,&苏志国.(2007).determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting.chinesejournalofanalyticalchemistry,35(1),43.
MLA	刘涛,et al."determinationofmodificationdegreeofsuccinylatedgelatinbysizeexclusionchromatographycoupledwithmultianglelaserlightscatting".chinesejournalofanalyticalchemistry 35.1(2007):43.