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浏览/检索结果: 共4条，第1-4条 帮助 已选(0)清除 条数/页：5101520253035404550556065707580859095100   排序方式：请选择作者升序作者降序题名升序题名降序发表日期升序发表日期降序提交时间升序提交时间降序 Elucidating the role of free polycationic chains in polycation gene carriers by free chains of polyethylenimine or N,N,N-trimethyl chitosan plus a certain polyplex 期刊论文 INTERNATIONAL JOURNAL OF NANOMEDICINE, 2014, 卷号: 9 作者:  Xu, Tao;  Liu, Wei;  Wang, Suhang;  Shao, Zhengzhong 收藏  |  浏览/下载：3/0  |  提交时间：2019/12/19 cellular uptake pathway  intracellular trafficking  gene delivery  transfection efficiency  mechanism   Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model 期刊论文 International Journal of Nanomedicine, 2013, 卷号: 8, 期号: 1, 页码: 3107-3118 作者:  Chen, Yan;  Yang, Lifang*;  Huang, Suping;  Li, Zhi;  Zhang, Lu 收藏  |  浏览/下载：8/0  |  提交时间：2019/12/03 DNAzymes are synthetic  single-stranded  catalytic nucleic acids that bind and cleave target mRNA in a sequence-specific manner  and have been explored for genotherapeutics. One bottleneck restricting their application is the lack of an efficient delivery system. As an inorganic nanomaterial with potentially wide application  nanohydroxyapatite particles (nHAP) have attracted increasing attention as new candidates for nonviral vectors. In this study  we developed an nHAP-based delivery system and explored its cellular uptake mechanisms  intracellular localization  and biological effects. Absorption of arginine-modified nanohydroxyapatite particles (Arg-nHAP) and DZ1 (latent membrane protein 1 [LMP1]-targeted) reached nearly 100% efficiency under in vitro conditions. Using specific inhibitors  cellular uptake of the Arg-nHAP/DZ1 complex was shown to be mediated by the energy-dependent endocytosis pathway. Further  effective intracellular delivery and nuclear localization of the complex was confirmed by confocal microscopy. Biologically  the complex successfully downregulated the expression of LMP1 in nasopharyngeal carcinoma cells. In a mouse tumor xenograft model  the complex was shown to be delivered efficiently to tumor tissue  downregulating expression of LMP1 and suppressing tumor growth. These results suggest that Arg-nHAP may be an efficient vector for nucleic acid-based drugs with potential clinical application.   Size-Dependent Stability of Water-Solubilized CdTe Quantum Dots and Their Uptake Mechanism by Live HeLa Cells 期刊论文 acs applied materials & interfaces, 2013, 卷号: 5, 期号: 4, 页码: 1190-1196 Wang TT; Jiang X 收藏  |  浏览/下载：13/0  |  提交时间：2014/04/16 IN-VIVO  PHOTOCHEMICAL INSTABILITY  GOLD NANOPARTICLES  CELLULAR UPTAKE  SURFACE-CHARGE  NANOCRYSTALS  TOXICITY  CLATHRIN  DELIVERY  PATHWAY   Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells 期刊论文 nanoscale, 2011, 卷号: 3, 期号: 5, 页码: 2028-2035 Jiang XE; Musyanovych A; Rocker C; Landfester K; Mailander V; Nienhaus GU 收藏  |  浏览/下载：17/0  |  提交时间：2012/06/11 RECEPTOR-MEDIATED ENDOCYTOSIS  COATED PIT FORMATION  GOLD NANOPARTICLES  CELLULAR UPTAKE  PROTEIN CORONA  HELA-CELLS  CLATHRIN  PATHWAY  SIZE  MECHANISM