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一种从霞水母刺丝囊毒素内分离致死毒素蛋白的方法 专利
专利类型: 发明, 专利号: CN201110027667.7, 申请日期: 2011-09-07, 公开日期: 2011-09-07
作者:  于华华;  邢荣娥;  李鹏程
收藏  |  浏览/下载:17/0  |  提交时间:2014/08/04
一种从霞水母刺丝囊毒素中分离致死毒素蛋白的方法  2)将上述步骤1)上清液于2~6℃下用缓冲液透析过夜  3)将步骤2)所得上清液过滤  4)将步骤3)得到的上清液用强阴离子交换树脂Mono?Q预装柱纯化分离  其特征在于:1)将霞水母刺丝囊细胞加入预冷缓冲液中破碎  然后低温离心  而后用含Con?A?Sepharose?4B亲和柱进行分离  依次采用pH7.8  破碎后2~6℃离心  收集上清液  分离时采用含有0.1~0.3Mα?甲基?D?甘露糖苷和0.1~0.3MNaCl的pH7.4  浓度为10~30mM的Tris?HCl缓冲液和含1M?NaCl的pH7.8  收集上清液  待用  浓度10~30mM的Tris?HCl缓冲液进行洗脱并收集洗脱液  浓度为10~30mM的Tris?HCl缓冲液进行梯度洗脱  待用  低温离心  流速为0.2~0.5mLmin?1  收集上清液  收集约25~40min洗脱液  待用  即为从霞水母刺丝囊毒素中分离致死毒素蛋白。  
Design and simulation of CMOS star sensor lens with large relative aperture and wide field (EI CONFERENCE) 会议论文
2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011, August 12, 2011 - August 14, 2011, Harbin, China
Zhong X.; Jin G.
收藏  |  浏览/下载:57/0  |  提交时间:2013/03/25
A 50mm focal length  F/1.25  Gaussian type optical system is designed in this paper  which has 20field of view. Actual star image adding optical cross-talk effect of CMOS(Complementary Metal Oxide Semiconductor)  detector is simulated by non-sequential ray tracing in ZEMAX optical design and analysis software to evaluate its performance. The distortion relative to image spot centriod is introduced and calculated by simulated star images  which is less than 0.03% of lens in this paper. Thermal adaptability is discussed by opt-mechanic thermal analysis  which shows the RMS spot radius of marginal filed changes less than 0.7m under 10C temperature gradient  and changes less than 1m during homogeneous temperature changing from 40C to 40C. The results of analyses and simulations show the design of this lens can achieve the requirement of high precision CMOS star sensor well. 2011 IEEE.  
北京市一次PM10污染过程模拟与气象诊断分析 期刊论文
2011, 卷号: 37, 期号: 11, 页码: 1743
作者:  黄青[1,2,3];  程水源[1];  苏福庆[4];  陈东升[1];  周颖[1]
收藏  |  浏览/下载:2/0  |  提交时间:2019/12/06
10815冷镦钢连铸坯的高温塑性 期刊论文
特殊钢, 2011, 卷号: 第32卷 第2期, 页码: 63-65
作者:  余刚;  袁武华;  刘泽亚;  傅强
收藏  |  浏览/下载:4/0  |  提交时间:2020/01/05


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