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	Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila
	Ma, Zhiguo1,2; Liu, Zhonghua1; Huang, Xun1
刊名	Development
	2010-11-15
卷号	137期号:22页码:3775-3784
关键词	Osbp Drosophila Sterol Spermatogenesis Individualization
ISSN号	0950-1991
DOI	10.1242/dev.049312
通讯作者	Huang, xun(xhuang@genetics.ac.cn)
英文摘要	Members of the oxysterol binding protein (osbp) family are involved in diverse biological processes, including non-vesicular sterol transport and vesicle trafficking. the mechanisms by which osbps integrate functionally with developmental and physiological processes remain elusive. here, we report the in vivo analysis of osbp function in the model organism drosophila. osbp mutants are male-sterile and exhibit defects in individualization, the process by which each spermatid is packaged into its own membrane. overexpression of osbp leads to post-eclosion behaviour defects that can be suppressed by co-expression of endoplasmic reticulum-specific vap family proteins. most notably, fan, a testis-specific vap protein, acts together with osbp genetically and physically to regulate the individualization process. osbp-positive and sterol-enriched speckles are found at the leading edge of the individualization complex in wild type but not in osbp or fan mutants, suggesting that sterol trafficking might play key roles during the membrane-remodelling phase of individualization. in addition, osbp mutants that are fed additional sterols partially recover fertility, implying that male sterility is attributable to sterol shortage. thus, we have identified an osbp- and fan-mediated sterol requirement in drosophila spermatogenesis.
WOS关键词	OXYSTEROL-BINDING-PROTEIN ; INTRACELLULAR CHOLESTEROL TRANSPORT ; LIPID-METABOLISM ; ENDS-OUT ; MEMBRANE ; INDIVIDUALIZATION ; GOLGI ; MELANOGASTER ; ACTIVATION ; HOMOLOGS
WOS研究方向	Developmental Biology
WOS类目	Developmental Biology
语种	英语
出版者	COMPANY OF BIOLOGISTS LTD
WOS记录号	WOS:000283671100006
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2408436
专题	中国科学院大学
通讯作者	Huang, Xun
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ma, Zhiguo,Liu, Zhonghua,Huang, Xun. Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila[J]. Development,2010,137(22):3775-3784.
APA	Ma, Zhiguo,Liu, Zhonghua,&Huang, Xun.(2010).Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila.Development,137(22),3775-3784.
MLA	Ma, Zhiguo,et al."Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila".Development 137.22(2010):3775-3784.

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