Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila | |
Ma, Zhiguo1,2; Liu, Zhonghua1; Huang, Xun1 | |
刊名 | Development
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2010-11-15 | |
卷号 | 137期号:22页码:3775-3784 |
关键词 | Osbp Drosophila Sterol Spermatogenesis Individualization |
ISSN号 | 0950-1991 |
DOI | 10.1242/dev.049312 |
通讯作者 | Huang, xun(xhuang@genetics.ac.cn) |
英文摘要 | Members of the oxysterol binding protein (osbp) family are involved in diverse biological processes, including non-vesicular sterol transport and vesicle trafficking. the mechanisms by which osbps integrate functionally with developmental and physiological processes remain elusive. here, we report the in vivo analysis of osbp function in the model organism drosophila. osbp mutants are male-sterile and exhibit defects in individualization, the process by which each spermatid is packaged into its own membrane. overexpression of osbp leads to post-eclosion behaviour defects that can be suppressed by co-expression of endoplasmic reticulum-specific vap family proteins. most notably, fan, a testis-specific vap protein, acts together with osbp genetically and physically to regulate the individualization process. osbp-positive and sterol-enriched speckles are found at the leading edge of the individualization complex in wild type but not in osbp or fan mutants, suggesting that sterol trafficking might play key roles during the membrane-remodelling phase of individualization. in addition, osbp mutants that are fed additional sterols partially recover fertility, implying that male sterility is attributable to sterol shortage. thus, we have identified an osbp- and fan-mediated sterol requirement in drosophila spermatogenesis. |
WOS关键词 | OXYSTEROL-BINDING-PROTEIN ; INTRACELLULAR CHOLESTEROL TRANSPORT ; LIPID-METABOLISM ; ENDS-OUT ; MEMBRANE ; INDIVIDUALIZATION ; GOLGI ; MELANOGASTER ; ACTIVATION ; HOMOLOGS |
WOS研究方向 | Developmental Biology |
WOS类目 | Developmental Biology |
语种 | 英语 |
出版者 | COMPANY OF BIOLOGISTS LTD |
WOS记录号 | WOS:000283671100006 |
内容类型 | 期刊论文 |
URI标识 | http://www.corc.org.cn/handle/1471x/2408436 |
专题 | 中国科学院大学 |
通讯作者 | Huang, Xun |
作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, Zhiguo,Liu, Zhonghua,Huang, Xun. Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila[J]. Development,2010,137(22):3775-3784. |
APA | Ma, Zhiguo,Liu, Zhonghua,&Huang, Xun.(2010).Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila.Development,137(22),3775-3784. |
MLA | Ma, Zhiguo,et al."Osbp- and fan-mediated sterol requirement for spermatogenesis in drosophila".Development 137.22(2010):3775-3784. |
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