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科研机构
大连化学物理研究所 [53]
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期刊论文 [38]
会议论文 [15]
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2019 [4]
2018 [2]
2017 [3]
2015 [5]
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物理化学 [19]
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专题:大连化学物理研究所
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A chemoenzymatic approach enables the site-specific conjugation of recombinant proteins
期刊论文
ELECTROPHORESIS, 2019, 卷号: 40, 期号: 16-17, 页码: 2125-2128
作者:
Wang, Yan
;
Dong, Mingming
;
Ye, Mingliang
收藏
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浏览/下载:69/0
  |  
提交时间:2019/12/02
Biorthogonal chemistry
Chemoenzymatic approach
Site-specific conjugation
Tyrosine phosphorylation
Characterization of the substrate scope of an alcohol dehydrogenase commonly used as methanol dehydrogenase
期刊论文
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2019, 卷号: 29, 期号: 12, 页码: 1446-1449
作者:
Feng, Yanbin
;
Guo, Xiaojia
;
Wang, Xueying
;
Liu, Yuxue
;
Liu, Wujun
收藏
  |  
浏览/下载:78/0
  |  
提交时间:2019/06/20
Alcohol dehydrogenase
Substrate preference
Methanol dehydrogenase
Geobacillus stearothermophilus
Molecular docking
Cofactor regeneration
Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein
期刊论文
JOURNAL OF SEPARATION SCIENCE, 2019, 卷号: 42, 期号: 3, 页码: 744-753
作者:
Qin, Qian
;
Wang, Bohong
;
Xu, Guowang
;
Xia, Tian
;
Lv, Wangjie
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  |  
浏览/下载:159/0
  |  
提交时间:2019/06/20
immobilized metal-ion affinity chromatography
layer-by-layer assembly
magnetic nanoparticles
protein purification
Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of his-tagged protein
期刊论文
Journal of separation science, 2019, 卷号: 42, 期号: 3, 页码: 744-753
作者:
Chang, Mengmeng
;
Qin, Qian
;
Wang, Bohong
;
Xia, Tian
;
Lv, Wangjie
收藏
  |  
浏览/下载:184/0
  |  
提交时间:2019/05/08
Immobilized metal-ion affinity chromatography
Layer-by-layer assembly
Magnetic nanoparticles
Protein purification
Tuning of acyl-ACP thioesterase activity directed for tailored fatty acid synthesis
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 7, 页码: 3173-3182
作者:
Feng, Yanbin
;
Zhang, Yunxiu
;
Wang, Yayue
;
Liu, Jiao
;
Liu, Yinghui
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  |  
浏览/下载:26/0
  |  
提交时间:2019/06/20
Acyl-acp Thioesterase
Medium-chain Fatty Acids
Fatty Acid Tailoring
Substrate Selectivity
Competitive Relationship
Structural Insights into the Thermophilic Adaption Mechanism of Endo-1,4-beta-Xylanase from Caldicellulosiruptor owensensis
期刊论文
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 卷号: 66, 期号: 1, 页码: 187-193
作者:
Liu, Xin
;
Liu, Tengfei
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浏览/下载:38/0
  |  
提交时间:2019/06/20
Gh10 Xylanase
Caldicellulosiruptor Owensensis
Thermostability
Crystal Structure
Loop 7
Surface sieving coordinated IMAC material for purification of His-tagged proteins
期刊论文
ANALYTICA CHIMICA ACTA, 2017, 卷号: 997, 页码: 9-15
作者:
Zhang, Lihua
;
Li, Senwu
;
Yang, Kaiguang
;
Liu, Lukuan
;
Zhao, Baofeng
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  |  
浏览/下载:180/0
  |  
提交时间:2017/12/19
Surface sieving
Metal ion affinity
Protein purification
His-tag
Surface sieving coordinated IMAC material for purification of His-tagged proteins
期刊论文
ANALYTICA CHIMICA ACTA, 2017, 卷号: 997, 页码: 9-15
作者:
Zhang, Lihua
;
Li, Senwu
;
Yang, Kaiguang
;
Liu, Lukuan
;
Zhao, Baofeng
收藏
  |  
浏览/下载:170/0
  |  
提交时间:2017/12/19
Surface sieving
Metal ion affinity
Protein purification
His-tag
Mixed-mode reversed phase/positively charged repulsion chromatography for intact protein separation
期刊论文
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 卷号: 138, 页码: 63-69
作者:
Ding, Ling
;
Guo, Zhimou
;
Hu, Zhuo
;
Liang, Xinmiao
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  |  
浏览/下载:14/0
  |  
提交时间:2017/10/29
Mixed-mode Stationary Phase
Reversed-phase Liquid Chromaitography
Positively Charged Repulsion
Intact Protein Separation
Click Chemistry
Stirring rate regulates the proliferation and metabolism of microencapsulated recombinant CHO cells
期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2015, 卷号: 62, 期号: 6, 页码: 833-839
作者:
Wang, Yu
;
Zhang, Ying
;
Li, Na
;
Chen, Li
;
Zhang, Demeng
收藏
  |  
浏览/下载:16/0
  |  
提交时间:2019/06/20
Cell Culture
Microencapsulated
Recombinant Protein Expression
Stirring Rate
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