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High hydrostatic pressure refolding of highly hydrophobic protein: A case study of recombinant human interferon beta-1b from inclusion bodies
期刊论文
BIOCHEMICAL ENGINEERING JOURNAL, 2021, 卷号: 172, 页码: 9
作者:
Wang, Qi
;
Zhang, Chun
;
Li, Zenglan
;
Guo, Fangxia
;
Zhang, Jing
收藏
  |  
浏览/下载:21/0
  |  
提交时间:2021/08/31
High hydrostatic pressure
Refolding
Hydrophobic protein
rhIFN beta-1b
Inclusion bodies
Microcalorimetric investigation of the ATPase activity and the refolding activity of GroEL system
期刊论文
Journal of Thermal Analysis and Calorimetry, 2019, 卷号: 135, 期号: 4, 页码: 2411-2418
作者:
Chen, Daixiong
;
Zhang, Ke
;
Li, Jiaokun
;
Zeng, Chi
;
Huang, Qiuxia
收藏
  |  
浏览/下载:14/0
  |  
提交时间:2019/12/27
Calorimetric
GroEL
Molecular chaperones
ATP hydrolysis
Refolding
Enzyme activity of Schistosoma japonicum cercarial elastase SjCE-2b ascertained by in vitro refolded recombinant protein
期刊论文
ACTA TROPICA, 2018, 卷号: 187
作者:
Zhang, Ting
;
Mo, Xiao-Jin
;
Xu, Bin
;
Yang, Zhong
;
Gobert, Geoffrey N.
收藏
  |  
浏览/下载:14/0
  |  
提交时间:2019/12/05
Schistosoma japonicum
Protein refolding
Cercarial elastase
Inclusion body
Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli
期刊论文
PROCESS BIOCHEMISTRY, 2017, 卷号: 62, 期号: 00, 页码: 80-90
作者:
Li, Zenglan
;
Zhang, Chun
;
Zhang, Yan
;
Liu, Yongdong
;
Li, Xiunan
收藏
  |  
浏览/下载:36/0
  |  
提交时间:2018/01/19
Recombinant Pertactin
Refolding
Aggregates
Dilution
High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2017, 卷号: 133, 页码: 152-159
作者:
Wang, Qi
;
Liu, Yongdong
;
Zhang, Chun
;
Guo, Fangxia
;
Feng, Cui
收藏
  |  
浏览/下载:20/0
  |  
提交时间:2017/07/10
rhCNTF
High hydrostatic pressure
Inclusion bodies
Hydrophobic aggregation
Refolding
A camelid nanobody against EGFR was easily obtained through refolding of inclusion body expressed in Escherichia coli
期刊论文
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2017, 卷号: 64, 页码: 895-901
作者:
Xu, Li
;
Song, Xiaoyu
;
Jia, Lingyun
收藏
  |  
浏览/下载:7/0
  |  
提交时间:2019/12/02
Arg
dilution refolding
EGFR
glutathione
inclusion body
nanobody
Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2016, 卷号: 122, 期号: JUNE, 页码: 1-7
作者:
Chen, Ying
;
Wang, Qi
;
Zhang, Chun
;
Li, Xiunan
;
Gao, Qiang
收藏
  |  
浏览/下载:24/0
  |  
提交时间:2016/07/15
Proinsulin
Inclusion bodies
Additive
Oxidative refolding
Diselenide
Aggregate
Nano-cage-mediated refolding of insulin by peg-pe micelle
期刊论文
Biomaterials, 2016, 卷号: 77, 页码: 139-148
作者:
Fang, Xiaocui
;
Yang, Tao
;
Wang, Luoyang
;
Yu, Jibing
;
Wei, Xiuli
收藏
  |  
浏览/下载:35/0
  |  
提交时间:2019/04/23
Insulin
Dtt
Refolding
Peg-pe micelle
Nano-cage
Negative charge layer
Recombinant expression, refolding, purification and characterization of Pseudomonas aeruginosa protease IV in Escherichia coli
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2016, 卷号: 126
作者:
Zhao, Mingzhi
;
Cai, Man
;
Wu, Feilin
;
Zhang, Yao
;
Xiong, Zhi
收藏
  |  
浏览/下载:7/0
  |  
提交时间:2019/12/05
Pseudomonas aeruginosa
Protease IV
Recombinant expression
Refolding
Purification
Activity
Optimization of dilution refolding conditions for a camelid single domain antibody against human beta-2-microglobulin
期刊论文
PROTEIN EXPRESSION AND PURIFICATION, 2016, 卷号: 117, 页码: 59-66
作者:
Bao, Xiaobo
;
Xu, Li
;
Lu, Xu
;
Jia, Lingyun
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/09
Beta-2-microglobulin
Single domain antibody
Dilution refolding
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