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福州大学 [2]
长春应用化学研究所 [2]
生物物理研究所 [1]
化学研究所 [1]
上海药物研究所 [1]
武汉病毒研究所 [1]
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期刊论文 [13]
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2020 [1]
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Ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide nanosheets for visual detection of alkaline phosphatase activity
期刊论文
SENSORS AND ACTUATORS B-CHEMICAL, 2020, 卷号: 302, 页码: 127176
作者:
Yang, Q
;
Wang, XY
;
Peng, HL
;
Arabi, M
;
Li, JH
收藏
  |  
浏览/下载:39/0
  |  
提交时间:2020/07/08
Alkaline phosphatase
Visual detection
Ratiometric fluorescence
Colorimetry
Dual-mode assay
Rapid and Reliable Detection of Alkaline Phosphatase by a Hot Spots Amplification Strategy Based on Well-Controlled Assembly on Single Nanoparticle
期刊论文
ACS APPLIED MATERIALS & INTERFACES, 2017, 卷号: 9, 期号: 35
作者:
Zeng, Yi
;
Ren, Jia-Qiang
;
Wang, Shao-Kai
;
Mai, Jia-Ming
;
Qu, Bing
收藏
  |  
浏览/下载:4/0
  |  
提交时间:2019/12/05
alkaline phosphatase
SERS
one-step clinical assay
rapid detection
test kit
self-assembly on single NP
A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition
期刊论文
BIOSENSORS & BIOELECTRONICS, 2016, 卷号: 83, 页码: 274-280
作者:
Tang, Cong
;
Qian, Zhaosheng
;
Huang, Yuanyuan
;
Xu, Jiamin
;
Ao, Hang
收藏
  |  
浏览/下载:20/0
  |  
提交时间:2017/01/18
Carbon quantum dots
Alkaline phosphatase (ALP) activity
Real-time fluorometric assay
Photoinduced electron transfer (PET)
Highly sensitive visual detection of Avian Influenza A (H7N9) virus based on the enzyme-induced metallization
期刊论文
BIOSENSORS & BIOELECTRONICS, 2016, 卷号: 79, 页码: 874-880
作者:
Zhang, Huifang
;
Ma, Xiaoming
;
Hu, Shuisheng
;
Lin, Yue
;
Guo, Longhua
收藏
  |  
浏览/下载:6/0
  |  
提交时间:2019/11/21
Enzyme-linked immunosorbent assay
Colorimetric detection
Alkaline phosphatase
Naked-eye readout
Gold nanoparticles
Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels
期刊论文
International Journal of Nanomedicine, 2014, 卷号: 9, 期号: 1, 页码: 2619-2626
作者:
Liu, Lin*
;
Xing, Yun
;
Zhang, Hui
;
Liu, Ruili
;
Liu, Huijing
收藏
  |  
浏览/下载:56/0
  |  
提交时间:2019/12/03
Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins
differing from other types of proteins
contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein
we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method
recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically
rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid–carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L-1 for rHuEPO detection was achieved. Other glycosylated and non-glycosylated proteins
such as horseradish peroxidase
prostate specific antigen
metallothionein
streptavidin
and thrombin showed no interference in the detection assay.
High-Resolution Colorimetric Assay for Rapid Visual Readout of Phosphatase Activity Based on Gold/Silver Core/Shell Nanorod
期刊论文
ACS APPLIED MATERIALS & INTERFACES, 2014, 卷号: 6, 页码: 18243-18250
作者:
Gao, Zhuangqiang
;
Deng, Kaichao
;
Wang, Xu-Dong
;
Miro, Manuel
;
Tang, Dianping
收藏
  |  
浏览/下载:3/0
  |  
提交时间:2019/11/21
high-resolution colorimetric assay
bare-eyes
alkaline phosphatase
localized surface plasmon resonance
gold nanorod
Substrate hydrolysis triggered formation of fluorescent gold nanoclusters - a new platform for the sensing of enzyme activity
期刊论文
chemical communications, 2013, 卷号: 49, 期号: 84, 页码: 9821-9823
Chen Y
;
Zhou HP
;
Wang Y
;
Li WY
;
Chen J
;
Lin Q
;
Yu C
收藏
  |  
浏览/下载:13/0
  |  
提交时间:2014/04/16
NANOPARTICLE AGGREGATION
ALKALINE-PHOSPHATASE
SENSITIVE DETECTION
COLORIMETRIC ASSAY
CLUSTERS
BIOCATALYSIS
NANOCRYSTALS
INHIBITION
ESTERASE
Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing
期刊论文
chemistry-a european journal, 2013, 卷号: 19, 期号: 38, 页码: 12846-12852
Wang Y
;
Chen J
;
Jiao HP
;
Chen Y
;
Li WY
;
Zhang QF
;
Yu C
收藏
  |  
浏览/下载:12/0
  |  
提交时间:2014/04/15
ALKALINE-PHOSPHATASE ACTIVITY
AGGREGATION-INDUCED EMISSION
CONJUGATED POLYELECTROLYTES
GOLD NANOPARTICLES
ASSAY
DNA
BIOCHEMISTRY
SENSORS
SILOLE
WATER
Development of a universal phosphorylated peptide-binding protein for simultaneous assay of kinases
期刊论文
BIOSENSORS & BIOELECTRONICS, 2009, 卷号: 24, 期号: 9, 页码: 2871-2877
Li, Wei
;
Bi, Lijun
;
毕利军
;
Wang, Wenhua
;
Li, Yongjin
;
Zhou, Yafeng
;
Wei, Hongping
;
Jiang, Tao
;
江涛
;
Bai, Lin
;
chen, yuanyuan
;
陈媛媛
;
Zhang, Zhiping
;
Yuan, Xinghua
;
Xiao, Jianping
;
Zhang, Xianen
;
张先恩
收藏
  |  
浏览/下载:34/0
  |  
提交时间:2013/12/25
Protein kinase assay
Alkaline phosphatase
Phosphorylated peptide-binding protein
Peptide chip
Cloning, expression, and functional analysis of human dopamine d1 receptors
期刊论文
Acta pharmacologica sinica, 2005, 卷号: 26, 期号: 1, 页码: 27-32
作者:
Sun, WC
;
Jin, L
;
Cao, Y
;
Wang, LZ
;
Meng, F
收藏
  |  
浏览/下载:46/0
  |  
提交时间:2019/05/09
Camp response element-binding protein
Alkaline phosphatase
Reporter genes
G-protein-coupled receptors
Dopamine d1 receptor
Radioligand assay
Calcium
Fluorescence screening
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