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Ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide nanosheets for visual detection of alkaline phosphatase activity 期刊论文
SENSORS AND ACTUATORS B-CHEMICAL, 2020, 卷号: 302, 页码: 127176
作者:  Yang, Q;  Wang, XY;  Peng, HL;  Arabi, M;  Li, JH
收藏  |  浏览/下载:39/0  |  提交时间:2020/07/08
Rapid and Reliable Detection of Alkaline Phosphatase by a Hot Spots Amplification Strategy Based on Well-Controlled Assembly on Single Nanoparticle 期刊论文
ACS APPLIED MATERIALS & INTERFACES, 2017, 卷号: 9, 期号: 35
作者:  Zeng, Yi;  Ren, Jia-Qiang;  Wang, Shao-Kai;  Mai, Jia-Ming;  Qu, Bing
收藏  |  浏览/下载:4/0  |  提交时间:2019/12/05
A fluorometric assay for alkaline phosphatase activity based on beta-cyclodextrin-modified carbon quantum dots through host-guest recognition 期刊论文
BIOSENSORS & BIOELECTRONICS, 2016, 卷号: 83, 页码: 274-280
作者:  Tang, Cong;  Qian, Zhaosheng;  Huang, Yuanyuan;  Xu, Jiamin;  Ao, Hang
收藏  |  浏览/下载:20/0  |  提交时间:2017/01/18
Highly sensitive visual detection of Avian Influenza A (H7N9) virus based on the enzyme-induced metallization 期刊论文
BIOSENSORS & BIOELECTRONICS, 2016, 卷号: 79, 页码: 874-880
作者:  Zhang, Huifang;  Ma, Xiaoming;  Hu, Shuisheng;  Lin, Yue;  Guo, Longhua
收藏  |  浏览/下载:6/0  |  提交时间:2019/11/21
Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels 期刊论文
International Journal of Nanomedicine, 2014, 卷号: 9, 期号: 1, 页码: 2619-2626
作者:  Liu, Lin*;  Xing, Yun;  Zhang, Hui;  Liu, Ruili;  Liu, Huijing
收藏  |  浏览/下载:56/0  |  提交时间:2019/12/03
Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins  differing from other types of proteins  contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein  we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method  recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically  rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid–carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L-1 for rHuEPO detection was achieved. Other glycosylated and non-glycosylated proteins  such as horseradish peroxidase  prostate specific antigen  metallothionein  streptavidin  and thrombin showed no interference in the detection assay.  
High-Resolution Colorimetric Assay for Rapid Visual Readout of Phosphatase Activity Based on Gold/Silver Core/Shell Nanorod 期刊论文
ACS APPLIED MATERIALS & INTERFACES, 2014, 卷号: 6, 页码: 18243-18250
作者:  Gao, Zhuangqiang;  Deng, Kaichao;  Wang, Xu-Dong;  Miro, Manuel;  Tang, Dianping
收藏  |  浏览/下载:3/0  |  提交时间:2019/11/21
Substrate hydrolysis triggered formation of fluorescent gold nanoclusters - a new platform for the sensing of enzyme activity 期刊论文
chemical communications, 2013, 卷号: 49, 期号: 84, 页码: 9821-9823
Chen Y; Zhou HP; Wang Y; Li WY; Chen J; Lin Q; Yu C
收藏  |  浏览/下载:13/0  |  提交时间:2014/04/16
Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing 期刊论文
chemistry-a european journal, 2013, 卷号: 19, 期号: 38, 页码: 12846-12852
Wang Y; Chen J; Jiao HP; Chen Y; Li WY; Zhang QF; Yu C
收藏  |  浏览/下载:12/0  |  提交时间:2014/04/15
Development of a universal phosphorylated peptide-binding protein for simultaneous assay of kinases 期刊论文
BIOSENSORS & BIOELECTRONICS, 2009, 卷号: 24, 期号: 9, 页码: 2871-2877
Li, Wei; Bi, Lijun; 毕利军; Wang, Wenhua; Li, Yongjin; Zhou, Yafeng; Wei, Hongping; Jiang, Tao; 江涛; Bai, Lin; chen, yuanyuan; 陈媛媛; Zhang, Zhiping; Yuan, Xinghua; Xiao, Jianping; Zhang, Xianen; 张先恩
收藏  |  浏览/下载:34/0  |  提交时间:2013/12/25
Cloning, expression, and functional analysis of human dopamine d1 receptors 期刊论文
Acta pharmacologica sinica, 2005, 卷号: 26, 期号: 1, 页码: 27-32
作者:  Sun, WC;  Jin, L;  Cao, Y;  Wang, LZ;  Meng, F
收藏  |  浏览/下载:46/0  |  提交时间:2019/05/09


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