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T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis 期刊论文
NUCLEIC ACIDS RESEARCH, 2019, 卷号: 47, 期号: 3
作者:  Xia, Yongzhen;  Li, Kai;  Li, Jingjing;  Wang, Tianqi;  Gu, Lichuan
收藏  |  浏览/下载:18/0  |  提交时间:2019/12/11
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis 期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 期号: 1
作者:  Wang, Junshu;  Liu, Fapeng;  Su, Tianyuan;  Chang, Yizhao;  Guo, Qi
收藏  |  浏览/下载:15/0  |  提交时间:2019/12/11
Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study 期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 7, 页码: 3217-3228
作者:  He, Chunjuan;  Yang, Yirui;  Zhao, Renfei;  Qu, Jingyao;  Jin, Lan
收藏  |  浏览/下载:4/0  |  提交时间:2019/12/11
Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids 期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 1, 页码: 249-260
作者:  Wu, Xiuyun;  Tian, Zhennan;  Jiang, Xukai;  Zhang, Qun;  Wang, Lushan
收藏  |  浏览/下载:5/0  |  提交时间:2019/12/11
Revised mechanism and improved efficiency of the quikchange site-directed mutagenesis method 期刊论文
Methods in Molecular Biology, 2017, 卷号: 1498, 页码: 367-374
作者:  Xia, Yongzhen;  Xun, Luying
收藏  |  浏览/下载:2/0  |  提交时间:2019/12/16
Development of a counterselectable seamless mutagenesis system in lactic acid bacteria 期刊论文
MICROBIAL CELL FACTORIES, 2017, 卷号: 16
作者:  Xin, Yongping;  Guo, Tingting;  Mu, Yingli;  Kong, Jian
收藏  |  浏览/下载:9/0  |  提交时间:2019/12/12
Exploring the N-terminal role of a heterologous protein in secreting out of Escherichia coli 期刊论文
BIOTECHNOLOGY AND BIOENGINEERING, 2016, 卷号: 113, 期号: 12, 页码: 2561-2567
作者:  Gao, Dongfang;  Luan, Yaqi;  Liang, Quanfeng;  Qi, Qingsheng
收藏  |  浏览/下载:2/0  |  提交时间:2019/12/16
Site-directed mutagenesis of alpha-L-rhamnosidase from Alternaria sp L1 to enhance synthesis yield of reverse hydrolysis based on rational design 期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 卷号: 100, 期号: 24, 页码: 10385-10394
作者:  Xu, Li;  Liu, Xiaohong;  Yin, Zhenhao;  Liu, Qian;  Lu, Lili
收藏  |  浏览/下载:9/0  |  提交时间:2019/12/16
Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp Y2 期刊论文
EXTREMOPHILES, 2016, 卷号: 20, 期号: 5, 页码: 631-639
作者:  Cui, Yanbing;  Cheng, Bin;  Meng, Yiwei;  Li, Chunfang;  Yin, Huijia
收藏  |  浏览/下载:5/0  |  提交时间:2019/12/16
Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo 期刊论文
Biotechnology and Bioengineering, 2015, 卷号: 112, 期号: 7, 页码: 1343-1353
作者:  Bian, Xiaoying;  Plaza, Alberto;  Yan, Fu;  Zhang, Youming;  Mueller, Rolf
收藏  |  浏览/下载:5/0  |  提交时间:2019/12/17


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