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科研机构
山东大学 [30]
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期刊论文 [30]
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2019 [2]
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T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
期刊论文
NUCLEIC ACIDS RESEARCH, 2019, 卷号: 47, 期号: 3
作者:
Xia, Yongzhen
;
Li, Kai
;
Li, Jingjing
;
Wang, Tianqi
;
Gu, Lichuan
收藏
  |  
浏览/下载:18/0
  |  
提交时间:2019/12/11
The phage T4 DNA ligase in vivo improves the survival-coupled bacterial mutagenesis
期刊论文
MICROBIAL CELL FACTORIES, 2019, 卷号: 18, 期号: 1
作者:
Wang, Junshu
;
Liu, Fapeng
;
Su, Tianyuan
;
Chang, Yizhao
;
Guo, Qi
收藏
  |  
浏览/下载:15/0
  |  
提交时间:2019/12/11
T4 DNA ligase
Microbial mutagenesis
Atmospheric and room temperature
plasma (ARTP)
Polyhydroxybutyrate (PHB)
Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 7, 页码: 3217-3228
作者:
He, Chunjuan
;
Yang, Yirui
;
Zhao, Renfei
;
Qu, Jingyao
;
Jin, Lan
收藏
  |  
浏览/下载:4/0
  |  
提交时间:2019/12/11
Levansucrase
Transfructosylation
Levan
Site-directed mutagenesis
Fructo-oligosaccharides
Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 卷号: 102, 期号: 1, 页码: 249-260
作者:
Wu, Xiuyun
;
Tian, Zhennan
;
Jiang, Xukai
;
Zhang, Qun
;
Wang, Lushan
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/11
GH11xylanase
Active site architecture
Rational design
Binding
affinity
Catalytic activity
Revised mechanism and improved efficiency of the quikchange site-directed mutagenesis method
期刊论文
Methods in Molecular Biology, 2017, 卷号: 1498, 页码: 367-374
作者:
Xia, Yongzhen
;
Xun, Luying
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/16
Overlap PCR
PCR
QuikChange
Site-directed mutagenesis
Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
期刊论文
MICROBIAL CELL FACTORIES, 2017, 卷号: 16
作者:
Xin, Yongping
;
Guo, Tingting
;
Mu, Yingli
;
Kong, Jian
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/12
Lactic acid bacteria
Temperature-sensitive plasmid
Seamless
mutagenesis
Counterselectable marker
pheS
Exploring the N-terminal role of a heterologous protein in secreting out of Escherichia coli
期刊论文
BIOTECHNOLOGY AND BIOENGINEERING, 2016, 卷号: 113, 期号: 12, 页码: 2561-2567
作者:
Gao, Dongfang
;
Luan, Yaqi
;
Liang, Quanfeng
;
Qi, Qingsheng
收藏
  |  
浏览/下载:2/0
  |  
提交时间:2019/12/16
heterologous signal sequence
random mutagenesis
secretion system
Escherichia coli
Site-directed mutagenesis of alpha-L-rhamnosidase from Alternaria sp L1 to enhance synthesis yield of reverse hydrolysis based on rational design
期刊论文
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 卷号: 100, 期号: 24, 页码: 10385-10394
作者:
Xu, Li
;
Liu, Xiaohong
;
Yin, Zhenhao
;
Liu, Qian
;
Lu, Lili
收藏
  |  
浏览/下载:9/0
  |  
提交时间:2019/12/16
Rhamnosidase
Reverse hydrolysis reaction
Site-directed mutagenesis
Rhamnose-containing chemicals
3D structural modeling
Expression and functional analysis of two NhaD type antiporters from the halotolerant and alkaliphilic Halomonas sp Y2
期刊论文
EXTREMOPHILES, 2016, 卷号: 20, 期号: 5, 页码: 631-639
作者:
Cui, Yanbing
;
Cheng, Bin
;
Meng, Yiwei
;
Li, Chunfang
;
Yin, Huijia
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/16
Na+/H+ antiporter
NhaD type homologues
Halomonas sp Y2
pH profile
Site-directed mutagenesis
Rational and efficient site-directed mutagenesis of adenylation domain alters relative yields of luminmide derivatives in vivo
期刊论文
Biotechnology and Bioengineering, 2015, 卷号: 112, 期号: 7, 页码: 1343-1353
作者:
Bian, Xiaoying
;
Plaza, Alberto
;
Yan, Fu
;
Zhang, Youming
;
Mueller, Rolf
收藏
  |  
浏览/下载:5/0
  |  
提交时间:2019/12/17
luminmide
point mutation
ccdB counterselection recombineering
biosynthesis
natural products
heterologous expression
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