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长春光学精密机械与物... [2]
复旦大学上海医学院 [2]
暨南大学 [1]
内容类型
会议论文 [5]
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2012 [2]
2009 [1]
2006 [1]
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DevelopmentandvalidationofanewUPLCmethodforvancomycinandcomparisontoFPIA(TDx)
会议论文
作者:
Yuran Cao
;
Jing Zhang
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浏览/下载:2/0
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提交时间:2019/12/19
ultra high performance liquid chromatography(UPLC)
vancomycin
fluorescence polarization immunoassay (FPIA)
therapeutic drug monitoring(TDM)
DevelopmentandvalidationofanewultraperformanceliquidchromatographicmethodforthequantificationofvancomycinandcomparisontoFPIA(TDx)assay
会议论文
作者:
Yuran Cao
;
Jing Zhang
;
Beining Guo
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浏览/下载:3/0
  |  
提交时间:2019/12/19
vancomycin
ultra high performance liquid chromatography
fluorescence polarization immunoassay
therapeutic drug monitoring
A sensitive solid-phase time-resolved fluorescence immunoassay apparatus (EI CONFERENCE)
会议论文
International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, June 17, 2009 - June 19, 2009, Beijing, China
Wang Y.-L.
;
Song K.-F.
;
Zhang W.-L.
;
Li J.-L.
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浏览/下载:25/0
  |  
提交时间:2013/03/25
In the device
a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source
and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA) [1]. The first one is to design optimum exciting optical system
so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least
the sample fluorescence through the integrating sphere and come to the grating monochromator
The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device
SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process
signal-to-noise ratio(SNR) will be improved
also adjust delay time
ampling frequency and sampling number arbitrarily. By testing
the sensitivity is 10-12mol/L(substance marked by Eu3+)
examination repeat is &le2.5%
examination linearity is from 10 -8mol/L to 10-12mol/L
correlation coefficient is 99.98%(p&le0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody
and solve the tumor
genetic variation
the virus protein detection. 2009 SPIE.
A study of interaction between FITC- labeled antibody and optical fiber surface (EI CONFERENCE)
会议论文
ICO20: Materials and Nanostructures, August 21, 2005 - August 26, 2005, Changchun, China
Chao K. F.
;
Zhang Y. L.
;
Kong X. G.
;
Zeng Q. H.
;
Liu R. L.
;
Wang X.
;
Feng L. Y.
;
Sun Y. J.
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浏览/下载:33/0
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提交时间:2013/03/25
The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper
FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore
red shift of the FITC fluorescence dye emission peak was observed on different fiber surface
exhibiting different interaction between FITC- labeled antibody and surface. Finally
under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.
Portable Quantitative Fluorescence Immunoassay Analyzer
会议论文
Guangtzhou, PEOPLES R CHINA, MAR 13-14, 2013
作者:
Xie, Mengyuan[1]
;
Zhang, Jun[1]
;
Gu, Wen[1]
;
Chen, Zhe[1]
;
Yu, Jianhui[1]
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提交时间:2019/12/17
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