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科研机构
中南林业科技大学 [1]
内容类型
期刊论文 [1]
发表日期
2010 [1]
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发表日期:2010
专题:中南林业科技大学
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Identification of a chitin deacetylase producing bacteria isolated from soil and its fermentation optimization
期刊论文
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, 2010, 卷号: 4, 期号: 23, 页码: 2597-2603
作者:
Zhou, Guoying*
;
Zhang, Huaiyun
;
He, Yuanhao
;
He, Li
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提交时间:2019/12/28
Chitin deacetylase (CDA) catalyzes the conversion of chitin to chitosan by the deacetylation of N-acetyl-D-glucosamine residues. In this study
9 strains of bacteria with chitin deacetylase activity were isolated from canal mud samples from theXiangjiang River in Changsha
Hunan Province. Of these 9 strains
the Z7 strain had the highest chitin deacetylase activity
as determined by enzyme assay screening. Based on its culture
morphological
physiological characteristics and molecular identification
strain Z7 was identified as Bacillus amyloliquefaciens. Yeast extract at a concentration of 1.0% was an ideal nitrogen source for production of CDA (16.78 Eu/ml). Starch at 2% functioned best as the major carbon source for optimum production of CDA (17.84 Eu/ml). Magnesium sulfate at a concentration of 0.04% was an ideal inorganic salt for CDA production (17.45 Eu/ml). Ideal pH and temperature for optimum production of CDA by this strain were pH 6 and 37˚C.
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