Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection

	Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection
	Wu, Hongliang 1,4; Li, Guoliang 2; Liu, Shucheng 3; Liu, Di 1,4; Chen, Guang 2; Hu, Na 1,4; Suo, Yourui 1; You, Jinmao 1,2
刊名	journal of pharmaceutical and biomedical analysis
	2015-03-25
卷号	107 页码:98-107
关键词	Triterpenic acids Pre-column derivatization High-performance liquid chromatography Ultrasound-assisted dispersive liquid-liquid microextraction Response surface methodology Traditional Chinese medicinal herbs
中文摘要	a novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) follow by high-performance liquid chromatography (hplc) with fluorescence detection. six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by ua-dllme using chloroform and acetone as the extraction and disperser solvents, respectively. after the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7h-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. the main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (rsm) combined with box-behnken design (bbd). the limits of detection (lods) and the limits of quantification (loqs) were in the range of 0.95-1.36 ng ml(-1) and 3.17-4.55 ng ml(-1), respectively. under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional chinese medicinal herbs. (c) 2014 elsevier b.v. all rights reserved.
英文摘要	a novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) follow by high-performance liquid chromatography (hplc) with fluorescence detection. six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by ua-dllme using chloroform and acetone as the extraction and disperser solvents, respectively. after the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7h-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. the main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (rsm) combined with box-behnken design (bbd). the limits of detection (lods) and the limits of quantification (loqs) were in the range of 0.95-1.36 ng ml(-1) and 3.17-4.55 ng ml(-1), respectively. under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional chinese medicinal herbs. (c) 2014 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]	chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]	chemistry ; pharmacology & pharmacy
关键词[WOS]	capillary-zone-electrophoresis ; thin-layer-chromatography ; solid-phase extraction ; ursolic acid ; oleanolic acid ; mass-spectrometry ; labeling reagent ; fatty-acids ; hplc ; derivatization
收录类别	SCI
语种	英语
WOS记录号	WOS:000351116900014
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5572]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection[J]. journal of pharmaceutical and biomedical analysis,2015,107:98-107.
APA	Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Liu, Di.,Chen, Guang.,...&You, Jinmao.(2015).Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection.journal of pharmaceutical and biomedical analysis,107,98-107.
MLA	Wu, Hongliang,et al."Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection".journal of pharmaceutical and biomedical analysis 107(2015):98-107.