Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting

	Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting
	Guo, Lijun 1; Xu, Kun 1; Liu, Zhiyuan 1; Zhang, Cunfang 1,2; Xin, Ying 1; Zhang, Zhiying 1
刊名	analytical biochemistry
	2015-06-01
卷号	478 页码:131-133
关键词	Cas9 CRISPR array CRISPR/Cas Multiplex targeting Streptococcus thermophilus
中文摘要	in addition to the advantages of scalable, affordable, and easy to engineer, the clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated protein (cas) technology is superior for multiplex targeting, which is laborious and inconvenient when achieved by cloning multiple grna expressing cassettes. here, we report a simple crispr array assembling method which will facilitate multiplex targeting usage. first, the streptococcus thermophilus crispr3/cas locus was cloned. second, different crispr arrays were assembled with different crrna spacers. transformation assays using different escherichia coli strains demonstrated efficient plasmid dna targeting, and we achieved targeting efficiency up to 95% with an assembled crispr array with three crrna spacers. (c) 2015 elsevier inc. all rights reserved.
英文摘要	in addition to the advantages of scalable, affordable, and easy to engineer, the clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated protein (cas) technology is superior for multiplex targeting, which is laborious and inconvenient when achieved by cloning multiple grna expressing cassettes. here, we report a simple crispr array assembling method which will facilitate multiplex targeting usage. first, the streptococcus thermophilus crispr3/cas locus was cloned. second, different crispr arrays were assembled with different crrna spacers. transformation assays using different escherichia coli strains demonstrated efficient plasmid dna targeting, and we achieved targeting efficiency up to 95% with an assembled crispr array with three crrna spacers. (c) 2015 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	cas systems ; vector ; immunity
收录类别	SCI
语种	英语
WOS记录号	WOS:000353839900020
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5551]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Guo, Lijun,Xu, Kun,Liu, Zhiyuan,et al. Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting[J]. analytical biochemistry,2015,478:131-133.
APA	Guo, Lijun,Xu, Kun,Liu, Zhiyuan,Zhang, Cunfang,Xin, Ying,&Zhang, Zhiying.(2015).Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting.analytical biochemistry,478,131-133.
MLA	Guo, Lijun,et al."Assembling the Streptococcus thermophilus clustered regularly interspaced short palindromic repeats (CRISPR) array for multiplex DNA targeting".analytical biochemistry 478(2015):131-133.