Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology

	Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology
	Wu, Hongliang 1,3; Li, Guoliang 2,4; Liu, Shucheng 4; Ji, Zhongyin 1,3; Zhang, Qiulong 1,3; Hu, Na 1,3; Suo, Yourui 1; You, Jinmao 1,2
刊名	food analytical methods
	2015-03-01
卷号	8 期号:3 页码:685-695
关键词	Biogenic amines Fluorescence labeling Ultrasound-assisted dispersive liquid-liquid microextraction Foodstuff samples Box-Behnken design
通讯作者	li, gl (reprint author), qufu normal univ, key lab life organ anal shandong prov, qufu, peoples r china.
中文摘要	a simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) was developed for the determination of biogenic amines (bas) in foodstuff samples by high-performance liquid chromatography (hplc) with fluorescence detection (fld). in this work, fluorescence probe 2-(11h-benzo[a]carbazol-11-yl) ethyl carbonochloridate (bcec-cl) was applied to label bas. what followed was the ua-dllme procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. a response surface methodology (rsm) based on a box-behnken design (bbd) was employed to optimize the main parameters affecting the fluorescence labeling and dllme efficiency. under the optimal conditions, this method offered low limits of detection (lods) of 1.1-7.8 ng/ml and limits of quantification (loqs) of 3.5-26.1 ng/ml. finally, the method was successfully used for the determination of trace bas in real samples and exhibited powerful potential in the high-throughput sample screening.
英文摘要	a simple, sensitive and selective method based on one-step fluorescence labeling and ultrasound-assisted dispersive liquid-liquid microextraction (ua-dllme) was developed for the determination of biogenic amines (bas) in foodstuff samples by high-performance liquid chromatography (hplc) with fluorescence detection (fld). in this work, fluorescence probe 2-(11h-benzo[a]carbazol-11-yl) ethyl carbonochloridate (bcec-cl) was applied to label bas. what followed was the ua-dllme procedure that was carried out using chloroform and acetone as extraction and disperser solvents, respectively. a response surface methodology (rsm) based on a box-behnken design (bbd) was employed to optimize the main parameters affecting the fluorescence labeling and dllme efficiency. under the optimal conditions, this method offered low limits of detection (lods) of 1.1-7.8 ng/ml and limits of quantification (loqs) of 3.5-26.1 ng/ml. finally, the method was successfully used for the determination of trace bas in real samples and exhibited powerful potential in the high-throughput sample screening.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	food science & technology
研究领域[WOS]	food science & technology
关键词[WOS]	chromatography-mass spectrometry ; capillary-electrophoresis ; ethyl chloroformate ; fish products ; derivatization ; identification ; reagent ; wines ; acids ; meat
收录类别	SCI
语种	英语
WOS记录号	WOS:000349553900018
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/5451]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China
推荐引用方式 GB/T 7714	Wu, Hongliang,Li, Guoliang,Liu, Shucheng,et al. Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology[J]. food analytical methods,2015,8(3):685-695.
APA	Wu, Hongliang.,Li, Guoliang.,Liu, Shucheng.,Ji, Zhongyin.,Zhang, Qiulong.,...&You, Jinmao.(2015).Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology.food analytical methods,8(3),685-695.
MLA	Wu, Hongliang,et al."Simultaneous Determination of Seven Biogenic Amines in Foodstuff Samples Using One-Step Fluorescence Labeling and Dispersive Liquid-Liquid Microextraction Followed by HPLC-FLD and Method Optimization Using Response Surface Methodology".food analytical methods 8.3(2015):685-695.