Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)

	Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)
	Chi, Liang; Liu, Qinghua; Xu, Shihong; Xiao, Zhizhong; Ma, Daoyuan; Li, Jun
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
	2015-10-01
卷号	188 期号:2015 页码:148-155
关键词	Turbot (Scophthalmus maximus) Trypsinogen/trypsin Maternal Development
英文摘要	Trypsin is an important serine protease that is considered to be involved in digestion of protein in teleost fish. Nevertheless, studies on trypsin/trypsinogen in fish embryos are very limited. In this study, the trypsinogen of turbot (Scophthalmus maximus) (tTG) was identified and the expression patterns and activity of trypsinogen/trypsin were investigated. The results showed that the tTG mRNA was evenly distributed in the oocytes and was also expressed along the yolk periphery in early embryos. At later embryo stages-and 1 days after hatching (dph), the tTG mRNA concentrated at the alimentary tract and head. Quantitative expression analysis showed that the tTG transcripts decreased after fertilization until the gastrula stage, then increased with the embryo and larvae development This result was also confirmed by the specific activity analysis of trypsin and in-situ-hybridization (ISH). All of the results indicated that tTG in early embryo stages was maternally derived and expressed by itself after gastrula stages. Additionally, location of tTG mRNA in embryos and larvae was investigated; we considered that trypsin may have multiple functions during the embryo development process. Based on our results regarding trypsinogen in embryos and early development, we concluded that the trypsin/trypsinogen in turbot embryos was inherited from a maternal source and we suggested that trypsin in early development has multiple functions in the process of development. (C) 2015 Elsevier Inc. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000360513400018
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/50426]
专题	海洋研究所_海洋生物技术研发中心
作者单位	Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chi, Liang,Liu, Qinghua,Xu, Shihong,et al. Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2015,188(2015):148-155.
APA	Chi, Liang,Liu, Qinghua,Xu, Shihong,Xiao, Zhizhong,Ma, Daoyuan,&Li, Jun.(2015).Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,188(2015),148-155.
MLA	Chi, Liang,et al."Maternally derived trypsin may have multiple functions in the early development of turbot (Scopthalmus maximus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 188.2015(2015):148-155.