H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin | |
Ji, ZS; Yuan, HZ; Liu, ML; Hu, JM | |
刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2002-08-22 | |
卷号 | 30期号:1页码:151-159 |
关键词 | ibuprofen human serum albumin acetonitrile NMR relaxation diffusion |
英文摘要 | The effect of acetonitrile (ACN) on the low-affinity interaction between human serum albumin (HSA) and ibuprofen (IBP) was studied using H-1-NMR techniques. Both chemical shift and relaxation measurements showed the addition of ACN to the solutions decreased the binding affinity of IBP to HSA and reduced the hydrophobic interaction between them. The self-diffusion coefficients of IBP were measured as a function of the drug concentration at different ACN concentrations. The association constant, K-a, for ligand-HSA complexes and the number of binding sites, n, are evaluated by the application of Langmuir isotherm. The results indicated that the value of n was about 38 without ACN, and about 26 with ACN concentration 12% (v/v%). The decreased binding capacity of IBP to HSA in the presence of ACN was mainly attributed to the competition of ACN with IBP to the low-affinity binding sites of HSA molecule. (C) 2002 Elsevier Science B.V. All rights reserved. |
WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
类目[WOS] | Chemistry, Analytical ; Pharmacology & Pharmacy |
研究领域[WOS] | Chemistry ; Pharmacology & Pharmacy |
关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; PROTEIN-BINDING ; LIQUID-CHROMATOGRAPHY ; CAPILLARY ELECTROPHORESIS ; AFFINITY-CHROMATOGRAPHY ; LIGAND-BINDING ; DIFFUSION ; NMR ; ENANTIOMERS ; SEPARATION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000178516900016 |
公开日期 | 2015-12-08 |
内容类型 | 期刊论文 |
源URL | [http://ir.wipm.ac.cn/handle/112942/8797] ![]() |
专题 | 武汉物理与数学研究所_2011年以前论文发表(包括2011年) |
作者单位 | 1.Wuhan Univ, Dept Anal Measurement Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China |
推荐引用方式 GB/T 7714 | Ji, ZS,Yuan, HZ,Liu, ML,et al. H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2002,30(1):151-159. |
APA | Ji, ZS,Yuan, HZ,Liu, ML,&Hu, JM.(2002).H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,30(1),151-159. |
MLA | Ji, ZS,et al."H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 30.1(2002):151-159. |
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