H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin
	Ji, ZS ; Yuan, HZ ; Liu, ML ; Hu, JM
刊名	JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
	2002-08-22
卷号	30 期号:1 页码:151-159
关键词	ibuprofen human serum albumin acetonitrile NMR relaxation diffusion
英文摘要	The effect of acetonitrile (ACN) on the low-affinity interaction between human serum albumin (HSA) and ibuprofen (IBP) was studied using H-1-NMR techniques. Both chemical shift and relaxation measurements showed the addition of ACN to the solutions decreased the binding affinity of IBP to HSA and reduced the hydrophobic interaction between them. The self-diffusion coefficients of IBP were measured as a function of the drug concentration at different ACN concentrations. The association constant, K-a, for ligand-HSA complexes and the number of binding sites, n, are evaluated by the application of Langmuir isotherm. The results indicated that the value of n was about 38 without ACN, and about 26 with ACN concentration 12% (v/v%). The decreased binding capacity of IBP to HSA in the presence of ACN was mainly attributed to the competition of ACN with IBP to the low-affinity binding sites of HSA molecule. (C) 2002 Elsevier Science B.V. All rights reserved.
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]	Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]	Chemistry ; Pharmacology & Pharmacy
关键词[WOS]	NUCLEAR-MAGNETIC-RESONANCE ; PROTEIN-BINDING ; LIQUID-CHROMATOGRAPHY ; CAPILLARY ELECTROPHORESIS ; AFFINITY-CHROMATOGRAPHY ; LIGAND-BINDING ; DIFFUSION ; NMR ; ENANTIOMERS ; SEPARATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000178516900016
公开日期	2015-12-08
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/8797]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Wuhan Univ, Dept Anal Measurement Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Ji, ZS,Yuan, HZ,Liu, ML,et al. H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2002,30(1):151-159.
APA	Ji, ZS,Yuan, HZ,Liu, ML,&Hu, JM.(2002).H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,30(1),151-159.
MLA	Ji, ZS,et al."H-1-NMR study of the effect of acetonitrile on the interaction of ibuprofen with human serum albumin".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 30.1(2002):151-159.