Binding site of Fe3+ at purine of ATP as studied by NMR

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	Binding site of Fe3+ at purine of ATP as studied by NMR
	Du, F ; Mao, XA ; Li, DF ; Liao, ZR
刊名	JOURNAL OF INORGANIC BIOCHEMISTRY
	2001-01-15
卷号	83 期号:2-3 页码:101-105
关键词	ATP-Fe3+ complex binding site NMR relaxation enhancement
英文摘要	The binding site of Fe3+ in the purine base of adenosine 5'-triphosphate (ATP) was studied by nuclear magnetic resonance (NMR). The NMR relaxation rates (R-1) of H-1 and P-31 in ATP solutions free of and containing ferric ions were measured in the pH range of 3-10. It was found that Fe3+ selectively enhanced the relaxation rate of protons. In the presence of Fe3+, the R-1 of H2 was much bigger than that of H8 at a lower pH (3-4.5), while at a higher pH (5.5-7.5) the R-1 of H8 was more enhanced than H2. At a pH of around 5, both H2 and H8, as well as all three phosphorous, showed a sudden jump in R-1. When pH>8, Fe3+ failed to show appreciable enhancement of R-1 to all protons and phosphorous. The quantitative data of relaxation rate enhancements suggest that the binding site of Fe in ATP is strongly dependent on pH. At lower pH values, Fe3+ binds N1 but at higher pH it binds to N7. When pH is around 5, the whole purine base donates the aromatic pi -electrons to the ferric ion, forming a ferrocene-like complex, while when pH>8, ATP could not form complexes with Fe3+. (C) 2001 Elsevier Science B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Inorganic & Nuclear
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	IRON ; COMPLEXES ; DNA ; AMP ; COORDINATION ; FE(III) ; P-31
收录类别	SCI
语种	英语
WOS记录号	WOS:000167256800004
公开日期	2015-12-01
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/8588]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, Lab NMR & Atom Mol Phys, Wuhan 430071, Peoples R China 2.Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA 3.Cent China Normal Univ, Dept Chem, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Du, F,Mao, XA,Li, DF,et al. Binding site of Fe3+ at purine of ATP as studied by NMR[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2001,83(2-3):101-105.
APA	Du, F,Mao, XA,Li, DF,&Liao, ZR.(2001).Binding site of Fe3+ at purine of ATP as studied by NMR.JOURNAL OF INORGANIC BIOCHEMISTRY,83(2-3),101-105.
MLA	Du, F,et al."Binding site of Fe3+ at purine of ATP as studied by NMR".JOURNAL OF INORGANIC BIOCHEMISTRY 83.2-3(2001):101-105.