Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera
	Wang, TZ ; Li, WP ; Wan, HW ; Zhou, PJ ; Qu, SS
刊名	JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
	2000-08-10
卷号	45 期号:1 页码:57-63
关键词	laccase laccase activity microcalorimetry substrate
英文摘要	The reactions between Laccase (extracted from China lacquer of Rhus vernicifera) and various substrates (3,4-Dihydroxybenzaldehyde, Guaiacol, Pyrogallol, Gallic acid) have been studied using LKB-2107 batch microcalorimetry system. Based on calorimetry, a new method has been proposed. Laccase activity and the Michaelis constant K-m have been determined simultaneously by this method. The method is simple, sample-saving, and valid for a wider range of substrate concentrations. Furthermore, it can be extended for assaying other enzymes catalyzing reactions using this method. (C) 2000 Elsevier Science B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	INTERMEDIATE ; REDUCTION ; DIOXYGEN
收录类别	SCI
语种	英语
WOS记录号	WOS:000088647700004
公开日期	2015-12-01
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/8554]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China 2.Acad Sinica, Wuhan Inst Phys & Math, Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, TZ,Li, WP,Wan, HW,et al. Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera[J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,2000,45(1):57-63.
APA	Wang, TZ,Li, WP,Wan, HW,Zhou, PJ,&Qu, SS.(2000).Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera.JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS,45(1),57-63.
MLA	Wang, TZ,et al."Assay for Laccase activity by microcalorimetry: Laccase was extracted from China lacquer of Rhus vernicifera".JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS 45.1(2000):57-63.