Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos

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	Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos
	He, Mu-Dan 1,2; Zhang, Feng-Hua 1,2; Wang, Hua-Lin 1,2; Wang, Hou-Peng 1; Zhu, Zuo-Yan 1; Sun, Yong-Hua 1
刊名	MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
	2015-10-01
卷号	780 页码:86-96
关键词	Double-strand breaks repair Transcription activator-like effector nuclease (TALEN) CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 Microhomology-mediated end joining (MMEJ) Ligase 3 Ligase 4
ISSN号	0027-5107
英文摘要	DNA double-strand break (DSB) repair is of considerable importance for genomic integrity. Homologous recombination (HR) and non-homologous end joining (NHEJ) are considered as two major mechanistically distinct pathways involved in repairing DSBs. In recent years, another DSB repair pathway, namely, microhomology-mediated end joining (MMEJ), has received increasing attention. MMEJ is generally believed to utilize an alternative mechanism to repair DSBs when NHEJ and other mechanisms fail. In this study, we utilized zebrafish as an in vivo model to study DSB repair and demonstrated that efficient MMEJ repair occurred in the zebrafish genome when DSBs were induced using TALEN (transcription activator-like effector nuclease) or CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 technologies. The wide existence of MMEJ repair events in zebrafish embryos was further demonstrated via the injection of several in vitro-designed exogenous MMEJ reporters. Interestingly, the inhibition of endogenous ligase 4 activity significantly increased MMEJ frequency, and the inhibition of ligase 3 activity severely decreased MMEJ activity. These results suggest that MMEJ in zebrafish is dependent on ligase 3 but independent of ligase 4. This study will enhance our understanding of the mechanisms of MMEJ in vivo and facilitate inducing desirable mutations via DSB-induced repair. (C) 2015 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
关键词[WOS]	GUIDED CAS9 NUCLEASE ; HOMOLOGOUS RECOMBINATION ; SACCHAROMYCES-CEREVISIAE ; VERTEBRATE CELLS ; KU80-DEFICIENT CELLS ; MAMMALIAN-CELLS ; GENE-EXPRESSION ; PATHWAY ; IV ; FISH
收录类别	SCI
语种	英语
WOS记录号	WOS:000362306000010
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27266]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Mu-Dan,Zhang, Feng-Hua,Wang, Hua-Lin,et al. Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2015,780:86-96.
APA	He, Mu-Dan,Zhang, Feng-Hua,Wang, Hua-Lin,Wang, Hou-Peng,Zhu, Zuo-Yan,&Sun, Yong-Hua.(2015).Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,780,86-96.
MLA	He, Mu-Dan,et al."Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS 780(2015):86-96.