Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations
	Yao, BL ; Wang, YL ; Hu, KS ; Chen, DL ; Zheng, Y ; Lei, M
刊名	photochemistry and photobiology
	2002-11-01
卷号	76 期号:5 页码:545-548
英文摘要	bacteriorhodopsin (br) films are oriented and deposited on indium tin oxide conductive glass by using electrophoretic sedimentation and langmuir-blodgett methods to construct sandwich-type photocells, respectively. the pulse response photoelectric signal of the br photocell under pulsed laser and the differential response photoelectric signal under irradiation of interval light are measured. the origins of these two types of photoelectric responses and their correlations are analyzed. the pulse response signal initiates from the ultrafast charge separation of the retinal and the proton translocation followed by the deprotonation and reprotonation of the schiff base and its surrounding amino acids. this is a quick response and is the preceding reaction of the differential response. the differential response signal is caused by the charging and discharging of the continuous proton current of the br light-driven proton pump at light-on and light-off, which is a slow process. the differential response is related to not only the construction of the br photocell but also the coupling mode of measurement. to observe the differential response signal, the br photocell must have large enough b-3 and b-3' components in its pulse response as well as an alternative coupling mode to measure it.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	light ; signals
收录类别	SCI
语种	英语
WOS记录号	WOS:000179368700013
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/25445]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt Technol, Xian, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Yao, BL,Wang, YL,Hu, KS,et al. Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations[J]. photochemistry and photobiology,2002,76(5):545-548.
APA	Yao, BL,Wang, YL,Hu, KS,Chen, DL,Zheng, Y,&Lei, M.(2002).Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations.photochemistry and photobiology,76(5),545-548.
MLA	Yao, BL,et al."Mechanisms of pulse response and differential response of bacteriorhodopsin and their relations".photochemistry and photobiology 76.5(2002):545-548.