Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography

	Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography
	Ding, Ling; Guo, Zhimou; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
刊名	journal of separation science
	2014-09-01
卷号	37 期号:18 页码:2467-2473
关键词	Alkyl chains Polar ligands Protein separation Retention equations Reversed-phase liquid chromatography
英文摘要	protein retention is very sensitive to the change of solvent composition in reversed-phase liquid chromatography for so called "on-off" mechanism, leading to difficulty in mobile phase optimization. in this study, a novel 3-chloropropyl trichlorosilane ligand bonded column was prepared for protein separation. the differences in retention characteristics between t he 3-chloropropyl trichlorosilane ligand bonded column and n-alkyl chainmodified (c-2, c-4, c-8) stationary phases were elucidated by the retention equation ln k = a + cc(b). retention parameters (a and c) of nine standard proteins with different molecular weights were calculated by using homemade software. results showed that retention times of nine proteins were similar on four columns, but the 3-chloropropyl trichlorosilane ligand bonded column obtained the lowest retention parameter values of larger proteins. it meant that their retention behavior affected by acetonitrile concentration would be different due to lower vertical bar c vertical bar values. more specifically, protein elution windows were broader, and retentions were less sensitive to the change of acetonitrile concentration on the 3-chloropropyl trichlorosilane ligand bonded column than that on other columns. meanwhile, the 3-chloropropyl trichlorosilane ligand bonded column displayed distinctive selectivity for some proteins. our results indicated that stationary phase with polar ligand provided potential solutions to the "on-off" problem and optimization in protein separation.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	gradient elution ; horizontal polymerization ; exchange chromatography ; retention equation ; intact proteins ; performance ; silica ; peptides ; packings ; hplc
收录类别	SCI
语种	英语
WOS记录号	WOS:000342909000002
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/143196]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,et al. Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography[J]. journal of separation science,2014,37(18):2467-2473.
APA	Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2014).Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography.journal of separation science,37(18),2467-2473.
MLA	Ding, Ling,et al."Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography".journal of separation science 37.18(2014):2467-2473.