Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair

	Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair
	Yao, Li 1; Yang, Jun 1; Guan, Ya-feng 1,2; Liu, Bai-zhan 3; Zheng, Sai-jing 3; Wang, Wei-miao 3; Zhu, Xiao-lan 1; Zhang, Zhi-dan 1
刊名	analytical and bioanalytical chemistry
	2012-11-01
卷号	404 期号:8 页码:2259-2266
关键词	4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol Molecularly imprinted polymers Solid-phase extraction Dispersive liquid-liquid microextraction Liquid chromatography tandem mass spectrometry Human hair
英文摘要	the tobacco-specific nitrosamine metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) is a valuable biomarker for human exposure to the carcinogenic nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) in tobacco and tobacco smoke. in this work, an efficient and sensitive method for the analysis of nnal in human hair was developed and validated. the hair sample was extracted by naoh solution digestion, purified by c-18 solid-phase extraction (spe) and molecularly imprinted solid-phase extraction, further enriched by reverse-phase ultrasound-assisted dispersive liquid-liquid microextraction (usa-dllme) into 1.0 % aqueous formic acid, and finally analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry. good linearity was obtained in the range of 0.24-10.0 pg/mg hair with a correlation coefficient of 0.9982, when 150 mg hair was analyzed. the limit of detection and lower limit of quantification were 0.08 and 0.24 pg/mg hair, respectively. accuracies determined from hair samples spiked with three different levels of nnal ranged between 87.3 and 107.7 %. intra- and inter-day relative standard deviations varied from 4.1 to 8.5 % and from 6.9 to 11.3 %, respectively. under the optimized conditions, an enrichment factor of 20 was obtained. finally, the developed method was applied for the analysis of nnal in smokers' hair. the proposed sample preparation procedure combining selectivity of two-step spe and enrichment of dllme significantly improves the purification and enrichment of the analyte and should be useful to analyze nnal in hair samples for cancer risk evaluation and cancer prevention in relation to exposure to the tobacco-specific carcinogen nnk.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	tobacco-specific nitrosamine ; solid-phase extraction ; molecularly imprinted polymer ; human urine ; selective extraction ; gas-chromatography ; organic-compounds ; in-vitro ; microextraction ; preconcentration
收录类别	SCI
语种	英语
WOS记录号	WOS:000310083900018
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142975]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Microinstrumentat & Analyt Chem, Dalian 116023, Peoples R China 3.China Natl Tobacco Corp, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China
推荐引用方式 GB/T 7714	Yao, Li,Yang, Jun,Guan, Ya-feng,et al. Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair[J]. analytical and bioanalytical chemistry,2012,404(8):2259-2266.
APA	Yao, Li.,Yang, Jun.,Guan, Ya-feng.,Liu, Bai-zhan.,Zheng, Sai-jing.,...&Zhang, Zhi-dan.(2012).Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair.analytical and bioanalytical chemistry,404(8),2259-2266.
MLA	Yao, Li,et al."Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair".analytical and bioanalytical chemistry 404.8(2012):2259-2266.