CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis

	CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis
	Huang, Yuejia 1,2; Wang, Wenwen 1; Yao, Phil 2; Wang, Xiwei 1,2; Liu, Xing 1,2; Zhuang, Xiaoxuan 1; Yan, Feng 1,2; Zhou, Jinhua 1; Du, Jian 1; Ward, Tarsha 2
刊名	journal of biological chemistry
	2012-01-06
卷号	287 期号:2 页码:1500-1509
英文摘要	mitotic chromosome segregation is orchestrated by the dynamic interaction of spindle microtubules with the kinetochore. although previous studies show that the mitotic kinesin cenp-e forms a link between attachment of the spindle microtubule to the kinetochore and the mitotic checkpoint signaling cascade, the molecular mechanism underlying dynamic kinetochore-microtubule interactions in mammalian cells remains elusive. here, we identify a novel interaction between cenp-e and skap that functions synergistically in governing dynamic kinetochore-microtubule interactions. skap binds to the c-terminal tail of cenp-e in vitro and is essential for an accurate kinetochore-microtubule attachment in vivo. immunoelectron microscopic analysis indicates that skap is a constituent of the kinetochore corona fibers of mammalian centromeres. depletion of skap or cenp-e by rna interference results in a dramatic reduction of inter-kinetochore tension, which causes chromosome mis-segregation with a prolonged delay in achieving metaphase alignment. importantly, skap binds to microtubules in vitro, and this interaction is synergized by cenp-e. based on these findings, we propose that skap cooperates with cenp-e to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation.
类目[WOS]	biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	microtubule plus ends ; mitotic checkpoint ; spindle microtubules ; kinetochore protein ; outer plate ; attachment ; localization ; motor ; aneuploidy ; complex
收录类别	SCI
语种	英语
WOS记录号	WOS:000299170300063
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142833]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230027, Peoples R China 2.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA 3.Dalian Inst Chem Phys, Dalian 116023, Peoples R China 4.Beijing Univ Chinese Med, Sch Grad Studies, Beijing 100029, Peoples R China 5.Guangzhou Inst Hlth Sci, Guangzhou 510515, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yuejia,Wang, Wenwen,Yao, Phil,et al. CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis[J]. journal of biological chemistry,2012,287(2):1500-1509.
APA	Huang, Yuejia.,Wang, Wenwen.,Yao, Phil.,Wang, Xiwei.,Liu, Xing.,...&Yao, Xuebiao.(2012).CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis.journal of biological chemistry,287(2),1500-1509.
MLA	Huang, Yuejia,et al."CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis".journal of biological chemistry 287.2(2012):1500-1509.