Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins

	Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins
	Liang, Yu 1,2; Zhu, Guijie 1,2; Wang, Tingting 1,2; Zhang, Xiaodan 1; Liang, Zhen 1; Zhang, Lihua 1; Zhang, Yukui 1
刊名	electrophoresis
	2011-10-01
卷号	32 期号:20 页码:2911-2914
关键词	Isoelectric focusing separation Monolithic immobilized pH gradient column Photografting Proteins
英文摘要	a new method was developed to prepare monolithic immobilized ph gradient (m-ipg) columns in uv-transparent fused-silica capillaries by the 5-min photopolymerization of acrylamide and n,n'-methylenebisacrylamide, followed by the 20-min photografting of the focused ampholine-derived glycidylmethacrylate monomer on the monolithic matrix, by which the preparation time was reduced, and the stability of the formed ph gradient was improved, compared with our previous methods. using the prepared m-ipg column, the baseline separation of proteins was achieved according to their pis. without carrier ampholytes added in the running buffer, the separated components could be detected with high sensitivity by uv at low wavelength.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	porous polymer monoliths ; capillary-zone-electrophoresis ; surface-chemistry ; chromatography ; electrochromatography ; digestion
收录类别	SCI
语种	英语
WOS记录号	WOS:000297424500019
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142699]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yu,Zhu, Guijie,Wang, Tingting,et al. Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins[J]. electrophoresis,2011,32(20):2911-2914.
APA	Liang, Yu.,Zhu, Guijie.,Wang, Tingting.,Zhang, Xiaodan.,Liang, Zhen.,...&Zhang, Yukui.(2011).Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins.electrophoresis,32(20),2911-2914.
MLA	Liang, Yu,et al."Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins".electrophoresis 32.20(2011):2911-2914.