Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis

	Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis
	Cai, Xiaoming; Wang, Chaoran; Xu, Junyan; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2011-03-15
卷号	879 期号:9-10 页码:657-661
关键词	Endogenous peptides Matrix solid-phase dispersion extraction NanoLC-MS/MS Peptide extraction Porcine hypothalamus
英文摘要	in this study, we investigated a novel application of matrix solid-phase dispersion (mspd) methodology for the extraction of endogenous peptides from porcine hypothalamus tissue samples. several experimental factors of the mspd procedure were examined. finally, silica-based octadecyl was chosen as dispersing material and blended with 0.25 g porcine hypothalamus at a ratio of 5, and 10 ml of 60% acetonitrile with 0.2% formic acid in water was chosen as the extraction and elution solvent. this mspd extraction method was compared to the classic acid extraction method. more peaks were observed in the mspd extracts (74 +/- 5) by maldi-tof ms than in acid extracts (34 +/- 5). moreover, 14 potential endogenous peptides were identified in the mspd extracts after nanolc-ms/ms analysis, while only 2 endogenous peptides in the acid extracts. these results indicated that mspd could be employed as a simple and efficient method for the extraction of endogenous peptides from tissues. (c) 2011 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	tandem mass-spectrometry ; liquid-chromatography ; neuropeptidomics ; optimization ; peptidomics ; discovery ; plasma ; ms/ms ; eggs
收录类别	SCI
语种	英语
WOS记录号	WOS:000288723500016
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142336]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Cai, Xiaoming,Wang, Chaoran,Xu, Junyan,et al. Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(9-10):657-661.
APA	Cai, Xiaoming,Wang, Chaoran,Xu, Junyan,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2011).Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(9-10),657-661.
MLA	Cai, Xiaoming,et al."Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.9-10(2011):657-661.