Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry

	Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry
	Dong Jun 1; Cai Xiao-ming 1; Zou Li-juan 2; Chen Cheng 1; Xue Xing-ya 1; Zhang Xiu-li 1; Liang Xin-miao 1
刊名	chemical research in chinese universities
	2011-09-01
卷号	27 期号:5 页码:750-755
关键词	Metabonomics Sample preparation Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF MS) Lung cancer Biomarker
英文摘要	centrifugal ultrafiltration after methanol extraction of whole plasma was used as an optimal condition for the preparation of blood plasma before metabonomic studies. the plasma samples from 102 lung cancer patients and 34 healthy volunteers were prepared with this approach. with ultra-performance liquid chromatography(uplc) coupled with quadrupole time-of-flight mass spectrometry(q-tof ms) analysis, the samples were investigated in order to find potential disease biomarkers. after data acquisition, orthogonal signal correction partial least squares models were built to differentiate the healthy volunteers from lung cancer patients and to identify metabolites that showed significantly different expression between the two groups. several metabolite ions were identified as potential biomarkers according to the variable importance in the project(vip) value in both ion modes. five lysophosphatidylcholines were further identified as specifically lysopc 16:0, isomer of lysopc 16:0, lysopc 18:0, lysopc 18:1 and lysopc 18:2. these results suggest that uplc coupled with q-tof ms is an effective technique for the analysis of plasma metabolites in metabonomic studies.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, multidisciplinary
研究领域[WOS]	chemistry
关键词[WOS]	early-diagnosis ; structural determination ; sample preparation ; human plasma ; uplc-ms ; metabonomics ; serum ; precipitation ; metabolites ; statistics
收录类别	SCI
语种	英语
WOS记录号	WOS:000296120800009
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142320]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Dong Jun,Cai Xiao-ming,Zou Li-juan,et al. Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry[J]. chemical research in chinese universities,2011,27(5):750-755.
APA	Dong Jun.,Cai Xiao-ming.,Zou Li-juan.,Chen Cheng.,Xue Xing-ya.,...&Liang Xin-miao.(2011).Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry.chemical research in chinese universities,27(5),750-755.
MLA	Dong Jun,et al."Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry".chemical research in chinese universities 27.5(2011):750-755.