Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column

	Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column
	Fu, Qing 1; Liang, Tu 2; Zhang, Xiuli 1; Du, Yuguang 1; Guo, Zhimou 1; Liang, Xinmiao 1,2
刊名	carbohydrate research
	2010-12-10
卷号	345 期号:18 页码:2690-2697
关键词	Oligosaccharides HILIC Click maltose Separation Purification
英文摘要	a general and efficient hydrophobic interaction liquid chromatography (hilic) method has been developed for the separation of various kinds of carbohydrates ranging from galactooligosaccharides carrageenan oligosaccharides sodium alginate chitooligosaccharides to higher molecular weight fructooligosaccharides three types of oligosaccharides (acidic basic and neutral) were purified by semi-preparative hilic as a result 18 oligosaccharides in high purity were obtained and their degrees of polymerization ranged from dp 2 to 7 furthermore oligosaccharides of plant origin were enriched and fractionated by solid-phase extraction (spe) on a graphitized carbon cartridge the oligosaccharide fractions that eluted from the cartridge were purified and then characterized by esims and nmr spectroscopy this strategy has led to the separation of a homogeneous raffinose family of oligosaccharides since the present method is universal efficient and stable separation and purification of various types of carbohydrates by hilic seem to be possible (c) 2010 elsevier ltd all rights reserved
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemistry & molecular biology ; chemistry, applied ; chemistry, organic
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	anion-exchange chromatography ; ionization mass-spectrometry ; solid-phase extraction ; graphitized carbon ; polar compounds ; oligosaccharides ; monosaccharides ; glycopeptides ; hplc ; derivatization
收录类别	SCI
语种	英语
WOS记录号	WOS:000285657700016
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142247]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China
推荐引用方式 GB/T 7714	Fu, Qing,Liang, Tu,Zhang, Xiuli,et al. Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column[J]. carbohydrate research,2010,345(18):2690-2697.
APA	Fu, Qing,Liang, Tu,Zhang, Xiuli,Du, Yuguang,Guo, Zhimou,&Liang, Xinmiao.(2010).Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column.carbohydrate research,345(18),2690-2697.
MLA	Fu, Qing,et al."Carbohydrate separation by hydrophilic interaction liquid chromatography on a 'click' maltose column".carbohydrate research 345.18(2010):2690-2697.