Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis

	Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis
	Liu, Hong-Tao 2,3; He, Jun-Lin 2; Li, Wen-Ming 2; Yang, Zhu 2; Wang, Ying-Xiong 2; Bai, Xue-Fang ; Yu, Chao 1,2; Du, Yu-Guang
刊名	carbohydrate polymers
	2010-05-16
卷号	80 期号:4 页码:1062-1071
关键词	Chitosan oligosaccharides Hydrogen peroxide Apoptosis Human umbilical vein endothelial cell
英文摘要	this study aimed to investigate the protective effect of chitosan oligosaccharides (cos) on human umbilical vein endothelial cell (huvec) apoptosis induced by hydrogen peroxide (h(2)o(2)). we found that cos not only reversed the decrease of cell viability and proliferation activity, but ameliorated nuclear chromatin damage in h(2)o(2)-induced huvecs. additionally, cos contributed to the decrease of cytosolic ca(2+) level, increase of mitochondrial membrane potential, up-regulation of bcl-2 mrna, down-regulation of bax mrna, reduction of cleaved caspase-3 protein, inhibition of phosphorylated p38 mitogen-activated protein kinase (mapk) and induction of phosphorylated akt in huvecs. further, h(2)o(2)-induced caspase-3 activation could be suppressed by p38 mapk inhibitor (sb203580) and up-regulated by phosphatidylinositol-3-kinase (pi3k) inhibitor (ly294002), indicating the involvement of p38 mapk and pi3k/akt signaling pathways. in conclusion, the results show that cos may effectively inhibit the h(2)o(2)-induced huvec apoptosis. (c) 2010 elsevier ltd. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, applied ; chemistry, organic ; polymer science
研究领域[WOS]	chemistry ; polymer science
关键词[WOS]	oxidative stress ; hippocampal-neurons ; up-regulation ; pc12 cells ; in-vitro ; chitooligosaccharides ; death ; activation ; survival ; bcl-2
收录类别	SCI
语种	英语
WOS记录号	WOS:000277636200008
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141795]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Nat Prod & Glyco Engn Res, Dalian 116023, Peoples R China 2.Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hong-Tao,He, Jun-Lin,Li, Wen-Ming,et al. Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis[J]. carbohydrate polymers,2010,80(4):1062-1071.
APA	Liu, Hong-Tao.,He, Jun-Lin.,Li, Wen-Ming.,Yang, Zhu.,Wang, Ying-Xiong.,...&Du, Yu-Guang.(2010).Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis.carbohydrate polymers,80(4),1062-1071.
MLA	Liu, Hong-Tao,et al."Chitosan oligosaccharides protect human umbilical vein endothelial cells from hydrogen peroxide-induced apoptosis".carbohydrate polymers 80.4(2010):1062-1071.