Systems toxicology study of doxorubicin on rats using ultra performance liquid chromatography coupled with mass spectrometry based metabolomics

	Systems toxicology study of doxorubicin on rats using ultra performance liquid chromatography coupled with mass spectrometry based metabolomics
	Wang, Jiangshan 1,2; Reijmers, Theo 2; Chen, Lijuan 4; Van der Heijden, Rob 2; Wang, Mei 3; Peng, Shuangqing 4; Hankemeier, Thomas 2; Xu, Guowang 1; Van der Greef, Jan 2,3
刊名	metabolomics
	2009-12-01
卷号	5 期号:4 页码:407-418
关键词	Metabolomics Doxorubicin UPLC-MS FTMS Systems toxicology
英文摘要	a metabolomics-based systems toxicology approach was used to profile the urinary metabolites for the toxicity related processes and pathogenesis induced by doxorubicin (dox) to rats. endogenous metabolite profiles were obtained with ultra performance liquid chromatography-mass spectrometry (uplc-ms) for rats receiving different single dosages of dox (5, 10 or 20 mg/kg) prior and at three time points after dosage. principal components analysis (pca) allowed detection of two major systemic metabolic changes with the time due to the induced toxicity. furthermore, analysis of variance (anova) simultaneous component analysis (asca) was applied to reveal the variation caused by time and dose, and their interaction in a multivariate way. finally, various metabolites involved in the toxic processes could be identified using their accurate mass and ms(n) experiments, and possible mechanisms of the toxicity of dox were postulated. in conclusion, metabolomics as a systems toxicology approach was able to provide comprehensive information on the dynamic process of drug induced toxicity. in addition, detection of the systemic toxic effects could be obtained with metabolomics at an earlier stage compared to the clinical chemistry and histopathological assessment.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	endocrinology & metabolism
研究领域[WOS]	endocrinology & metabolism
关键词[WOS]	mitochondrial toxicity ; h-1-nmr spectroscopy ; hplc-tof/ms ; metabonomics ; nephrotoxicity ; identification ; biology ; urine ; cardiomyopathy ; hepatotoxicity
收录类别	SCI
语种	英语
WOS记录号	WOS:000272849800003
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141507]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands 3.TNO, Qual Life & BioMed, NL-3700 AJ Zeist, Netherlands 4.Acad Mil Med Sci, Beijing Inst Dis Control & Prevent, Res & Evaluat Ctr Toxicol, Beijing 100071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jiangshan,Reijmers, Theo,Chen, Lijuan,et al. Systems toxicology study of doxorubicin on rats using ultra performance liquid chromatography coupled with mass spectrometry based metabolomics[J]. metabolomics,2009,5(4):407-418.
APA	Wang, Jiangshan.,Reijmers, Theo.,Chen, Lijuan.,Van der Heijden, Rob.,Wang, Mei.,...&Van der Greef, Jan.(2009).Systems toxicology study of doxorubicin on rats using ultra performance liquid chromatography coupled with mass spectrometry based metabolomics.metabolomics,5(4),407-418.
MLA	Wang, Jiangshan,et al."Systems toxicology study of doxorubicin on rats using ultra performance liquid chromatography coupled with mass spectrometry based metabolomics".metabolomics 5.4(2009):407-418.