Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry

	Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry
	Kong, Hongwei 1,2; Wang, Mei 3,4; Venema, Koen 4; Maathuis, Annet 4; van der Heijden, Rob 1; van der Greef, Jan 1,4; Xu, Guowang 2; Hankemeier, Thomas 1
刊名	journal of chromatography a
	2009-03-13
卷号	1216 期号:11 页码:2195-2203
关键词	Panax ginseng HPLC-FTICR-MS Ginsenoside Intestinal bacteria Metabolism TNO gastro-intestinal model (TIM) Acidic hydrolysis Metabolite profiling
英文摘要	a high-performance liquid chromatography-high resolution fourier transform ion cyclotron resonance mass spectrometry (hplc-fticr-ms) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. the metabolites were identified by high-resolution tandem mass spectrometry. degradation and bioconversion routes of the different ginsenosides at acidic (gastric) conditions and in the presence of intestinal microbiota were elaborated. besides hydrolysis (deglycosylation) also hydration reactions occurred at acidic conditions. the results illustrate the value of metabolite profiling by hplc-fticr-ms for understanding of the mechanisms in bioavailability of herbal drugs and their metabolites. (c) 2008 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	human intestinal bacteria ; panax-ginseng ; ginsenosides ; metabolism ; identification ; activation ; roots ; rh-2 ; rb-1 ; rat
收录类别	SCI
语种	英语
WOS记录号	WOS:000264226500030
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141396]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 3.SU BioMed, NL-3700 AJ Zeist, Netherlands 4.TNO Netherlands Org Appl Sci Res, Leiden, Netherlands
推荐引用方式 GB/T 7714	Kong, Hongwei,Wang, Mei,Venema, Koen,et al. Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry[J]. journal of chromatography a,2009,1216(11):2195-2203.
APA	Kong, Hongwei.,Wang, Mei.,Venema, Koen.,Maathuis, Annet.,van der Heijden, Rob.,...&Hankemeier, Thomas.(2009).Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry.journal of chromatography a,1216(11),2195-2203.
MLA	Kong, Hongwei,et al."Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry".journal of chromatography a 1216.11(2009):2195-2203.