Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS | |
Yuan, Huiming1,2; Zhou, Yuan1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1 | |
刊名 | journal of chromatography a |
2009-10-30 | |
卷号 | 1216期号:44页码:7478-7482 |
关键词 | Column switch recycling size exclusion chromatography Immobilized trypsin microenzymatic reactor mu RPLC-ESI-MS/MS Protein analysis |
英文摘要 | an integrated platform with the combination of proteins and peptides separation was established via the unit of on-line proteins digestion, by which proteins were in sequence separated by column switch recycling size exclusion chromatography (csrsec), on-line digested by an immobilized trypsin microreactor, trapped and desalted by two parallel c8 precolumns, separated by mu rplc with the linear gradient of organic modifier concentration, and identified by esi-ms/ms a 6-protein mixture. with mr ranging from 10 kda to 80 kda. was used to evaluate the performance of the integrated platform, and all proteins were identified with sequence coverage over 5.67%. our experimental results demonstrate that such an integrated platform is of advantages such as good time compatibility, high peak capacity, and facile automation. which might be a promising approach for proteome study (c) 2009 elsevier b.v. all rights reserved. |
WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
类目[WOS] | biochemical research methods ; chemistry, analytical |
研究领域[WOS] | biochemistry & molecular biology ; chemistry |
关键词[WOS] | human plasma proteome ; liquid-chromatography ; identification ; online ; technology ; digestion |
收录类别 | SCI ; ISTP |
语种 | 英语 |
WOS记录号 | WOS:000271250500021 |
公开日期 | 2015-11-17 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/141382] |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,et al. Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS[J]. journal of chromatography a,2009,1216(44):7478-7482. |
APA | Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2009).Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS.journal of chromatography a,1216(44),7478-7482. |
MLA | Yuan, Huiming,et al."Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS".journal of chromatography a 1216.44(2009):7478-7482. |
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