Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging

	Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging
	Wu, Jing 1; Ye, Zhiqiang 2; Wang, Guilan 2; Jin, Dayong 3; Yuan, Jingli 1,2; Guan, Yafeng 1; Piper, James 3
刊名	journal of materials chemistry
	2009
卷号	19 期号:9 页码:1258-1264
英文摘要	time-gated luminescence bioimaging based on microsecond-lifetime luminescent biolabels can provide complete background-free conditions for detecting target cells in an autofluorescence biosample matrix. however, a major drawback of the current lanthanide biolabels is the requirement for uv excitation (<370 nm), which leads to damage to many biological systems and greatly affects the improvement of time-gated luminescence instruments. herein we describe luminescent europium nanoparticles that have an excitation peak around 406 nm with high quantum yield (similar to 66%) and fine monodispersity in aqueous solutions. the nanoparticles were prepared by copolymerization of a visible-light-sensitized eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl) chlorosulfo-o-terphenyl-eu(3+)-2-(n,n-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine conjugated with 3-aminopropyl(triethoxy) silane, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion. characterization by transmission electron microscopy and luminescence spectroscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, <50 nm in diameter, and show strong visible-light-sensitized luminescence with a large quantum yield and a long luminescence lifetime. the new nanoparticles were successfully applied to distinguish an environmental pathogen, giardia lamblia, within a concentrate of environmental water sample using a time-gated luminescence microscope with pulsed visible light excitation. the method resulted in highly specific and sensitive imaging for giardia lamblia. these results suggest a broad range of potential bioimaging applications where both long time microscopy observation and high signal-to-background ratio are required for samples containing high concentrations of autofluorescence background.
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, physical ; materials science, multidisciplinary
研究领域[WOS]	chemistry ; materials science
关键词[WOS]	resolved fluorometric application ; doped silica nanoparticles ; prostate-specific antigen ; bioconjugated nanoparticles ; fluorescent nanoparticles ; cryptosporidium-parvum ; quantum dots ; chelate ; excitation ; microscopy
收录类别	SCI
语种	英语
WOS记录号	WOS:000263450300007
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/141273]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China 3.Macquarie Univ, Div Informat & Commun Sci, MQ Photon Ctr, N Ryde, NSW 2109, Australia
推荐引用方式 GB/T 7714	Wu, Jing,Ye, Zhiqiang,Wang, Guilan,et al. Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging[J]. journal of materials chemistry,2009,19(9):1258-1264.
APA	Wu, Jing.,Ye, Zhiqiang.,Wang, Guilan.,Jin, Dayong.,Yuan, Jingli.,...&Piper, James.(2009).Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging.journal of materials chemistry,19(9),1258-1264.
MLA	Wu, Jing,et al."Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging".journal of materials chemistry 19.9(2009):1258-1264.