Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography

	Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography
	Tian, Hongzhe; Xu, Jing; Guan, Yafeng
刊名	talanta
	2007-04-30
卷号	72 期号:2 页码:813-818
关键词	axial temperature gradient high temperature liquid chromatography micro-HPLC polycyclic aromatic hydrocarbons
英文摘要	the effect of axial temperature gradient (atg) along a microcolumn on the separation performance at both isocratic and gradient elution mode was investigated. a thermostat system was designed to form an atg along the packed column. polycyclic aromatic hydrocarbons (pahs) were separated on a 0.53 mm x 150 mm i.d. 5 mu m c-18 microcolumn, with water and acetonitrile as mobile phase. the separation results obtained at mobile phase gradient (mpg) and atg in microcolumn hplc were compared with the results performed at ambient conditions. extrapolated curves of peak width at half height (w(h)) versus ink showed that w(h) is narrower at the same retention time when atg was applied in addition to mpg. the column efficiency was enhanced 20-30% and the resolution was slightly reduced because of reduction of selectivity at elevated temperature at atg condition. the rsd of retention time in atg mode was less than 2.5%. (c) 2006 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	packed capillary columns ; hplc ; optimization ; separation ; retention
收录类别	SCI
语种	英语
WOS记录号	WOS:000246321200070
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140589]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Sci, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Tian, Hongzhe,Xu, Jing,Guan, Yafeng. Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography[J]. talanta,2007,72(2):813-818.
APA	Tian, Hongzhe,Xu, Jing,&Guan, Yafeng.(2007).Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography.talanta,72(2),813-818.
MLA	Tian, Hongzhe,et al."Axial temperature gradient and mobile phase gradient in microcolumn high-performance liquid chromatography".talanta 72.2(2007):813-818.