Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase

	Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase
	Li, Ning ; Zong, Min-Hua ; Liu, Xiu-Mei ; Ma, Ding
刊名	journal of molecular catalysis b-enzymatic
	2007-06-01
卷号	47 期号:1-2 页码:6-12
关键词	Pseudomonas cepacia lipase floxuridine 3 '-O-caproyl-floxuridine regioselective acylation vinyl caproate
英文摘要	3'-o-caproyl-floxuridine was prepared successfully through an enzymatic approach. among the seven commercially available lipases tested, pseudomonas cepacia lipase displayed the highest activity and regioselectivity towards the acylation of 3'-hydroxyl of floxuridine. effects of various parameters on the acylation reaction catalyzed by p. cepacia lipase were investigated systematically. the best reaction medium was found to be acetone or acetonitrile. the optimal enzyme dosage, molar ratio of vinyl caproate to floxuridine, and reaction temperature were 40 u, 7.5, and 35 c, respectively. under the optimal conditions, a substrate conversion of >99% and a regioselectivity of 93.5% could be achieved after a reaction time of 4 h. the half life of p. cepacia lipase was less than 4 batches with acetone or acetonitrile as the solvent. (c) 2007 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemistry & molecular biology ; chemistry, physical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	candida-antarctica lipase ; acid ester prodrugs ; colorectal-cancer ; organic-solvents ; acylation ; 5-fluoro-2'-deoxyuridine ; 1-beta-d-arabinofuranosylcytosine ; media ; immobilization ; 5-fluorouracil
收录类别	SCI
语种	英语
WOS记录号	WOS:000247432000002
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140576]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.S China Univ Technol, Lab Appl Biocatalysis, Guangzhou 510640, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Li, Ning,Zong, Min-Hua,Liu, Xiu-Mei,et al. Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase[J]. journal of molecular catalysis b-enzymatic,2007,47(1-2):6-12.
APA	Li, Ning,Zong, Min-Hua,Liu, Xiu-Mei,&Ma, Ding.(2007).Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase.journal of molecular catalysis b-enzymatic,47(1-2),6-12.
MLA	Li, Ning,et al."Regioselective synthesis of 3 '-O-caproyl-floxuridine catalyzed by Pseudomonas cepacia lipase".journal of molecular catalysis b-enzymatic 47.1-2(2007):6-12.