Enantioseparation of tetrahydropalmatine and Troger's base by molecularly imprinted monolith in capillary electrochromatography

	Enantioseparation of tetrahydropalmatine and Troger's base by molecularly imprinted monolith in capillary electrochromatography
	Ou, Junjie; Dong, Jing; Tian, Tuijun; Hu, Jiwei; Ye, Mingliang; Zou, Hanfa
刊名	journal of biochemical and biophysical methods
	2007-02-23
卷号	70 期号:1 页码:71-76
关键词	tetrahydropalmatine Troger's base molecular imprinting polymer capillary monolithic column chiral separation capillary electrochromatography
英文摘要	two chiral compounds, troger's base and tetrahydropalmatine, were enantioseparated on the (5s, 11s)-(-)-troger's base and l-tetrahydropalmatine imprinted monolithic capillary columns with cec, respectively. the monoliths were prepared by in situ thermal-initiated copolymerization of methacrylic acid (maa) and ethylene dimethacrylate (edma). after optimizing the ratio of porogens (toluene and dodecanol), the obtained monolithic capillary columns show good flow-through property and enantioselectivity. the influences of cec parameters such as ph of the buffer, organic solvent and salt concentration on the electroosmotic flow (eof) and recognition selectivity were systematically investigated. under the optimal conditions, baseline resolutions of two chiral compounds were achieved. in addition, the fast separation was obtained within 4 min by applying higher voltage and assisting pressure of 6 bar. (c) 2006 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	stationary phases ; dl-tetrahydropalmatine ; liquid-chromatography ; enantiomer separation ; chiral separations ; polymer-films ; column ; silica ; electrophoresis ; selectivity
收录类别	SCI
语种	英语
WOS记录号	WOS:000245001100010
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140543]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ou, Junjie,Dong, Jing,Tian, Tuijun,et al. Enantioseparation of tetrahydropalmatine and Troger's base by molecularly imprinted monolith in capillary electrochromatography[J]. journal of biochemical and biophysical methods,2007,70(1):71-76.
APA	Ou, Junjie,Dong, Jing,Tian, Tuijun,Hu, Jiwei,Ye, Mingliang,&Zou, Hanfa.(2007).Enantioseparation of tetrahydropalmatine and Troger's base by molecularly imprinted monolith in capillary electrochromatography.journal of biochemical and biophysical methods,70(1),71-76.
MLA	Ou, Junjie,et al."Enantioseparation of tetrahydropalmatine and Troger's base by molecularly imprinted monolith in capillary electrochromatography".journal of biochemical and biophysical methods 70.1(2007):71-76.