Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods

	Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods
	Su, Xingye; Qin, Feng; Kong, Liang; Ou, Junjie; Xie, Chuanhui; Zou, Hanfa
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2007
卷号	845 期号:1 页码:174-179
关键词	tetrahydropalmatine enantioselectivity DNA binding natural products chromatographic methods
英文摘要	a racemate from natural product, tetrahydropalmatine (thp), was characterized on its enantioselective binding to dna by the chromatographic methods including microdialysis/hplc, centrifugal ultrifiltration/hplc and immobilized dna affinity chromatography. it was found that its (+)-enantiomer was preferential to binding on b-form duplex dna including calf thymus dna, at and gc sequence oligo dna, as well as triplex oligo dna. the binding constants of the thp enantiomers to ct-dna were determined with the methods of microdialysis/hplc and frontal affinity chromatography. in addition, the dna structural preference of either enantiomer was evaluated with the chromatographic methods. (c) 2006 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; affinity-chromatography ; protein interactions ; metal-complexes ; topoisomerase-i ; microdialysis ; alkaloids ; ligands ; phases ; hplc
收录类别	SCI
语种	英语
WOS记录号	WOS:000243704200021
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140426]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Su, Xingye,Qin, Feng,Kong, Liang,et al. Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2007,845(1):174-179.
APA	Su, Xingye,Qin, Feng,Kong, Liang,Ou, Junjie,Xie, Chuanhui,&Zou, Hanfa.(2007).Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods.journal of chromatography b-analytical technologies in the biomedical and life sciences,845(1),174-179.
MLA	Su, Xingye,et al."Characterization of enantio selective binding of racemic natural tetrahydropalmatine to DNA by chromatographic methods".journal of chromatography b-analytical technologies in the biomedical and life sciences 845.1(2007):174-179.