Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine

	Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine
	Tian, Hongzhe; Yang, Bingcheng; Xu, Jing; Wang, Hua; Guan, Yafeng
刊名	journal of separation science
	2006-11-01
卷号	29 期号:17 页码:2692-2696
关键词	capillary LC-MS daidzein isoflavonoid pueraria radix puerarin
英文摘要	a specific lg-ms method was developed that allowed simultaneous determination of puerarin (pu) and its major metabolite, daidzein (da), in human urine samples. pu and da were separated on a packed capillary ods column with on column concentration. identification and quantification of the analytes were performed with esi-q-tof mass spectroscopy in negative ionization mode. the method was validated, yielding calibration curves with correlation coefficients greater than 0.998. the loq for pu and da from human urine samples was 0.1 and 0.05 nmol/ml, respectively. assay accuracy and precision of quality control samples were within +/- 15%. recoveries of pu and da in spiked samples were in the range of 79.6-90.4 and 82.3-92.4%, respectively.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	time-resolved fluoroimmunoassay ; electrochemical detection ; capillary-electrophoresis ; quantitative-analysis ; daidzein ; genistein ; isoflavones ; performance ; plasma ; soy
收录类别	SCI
语种	英语
WOS记录号	WOS:000243338600017
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/140270]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Tian, Hongzhe,Yang, Bingcheng,Xu, Jing,et al. Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine[J]. journal of separation science,2006,29(17):2692-2696.
APA	Tian, Hongzhe,Yang, Bingcheng,Xu, Jing,Wang, Hua,&Guan, Yafeng.(2006).Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine.journal of separation science,29(17),2692-2696.
MLA	Tian, Hongzhe,et al."Liquid chromatography-mass spectrometric analysis of puerarin and its metabolite in human urine".journal of separation science 29.17(2006):2692-2696.