A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity

	A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity
	Wang, JH ; Jing, K ; Wei, L ; Molchanova, V ; Chikalovets, I ; Belogortseva, N ; Luk'yanov, P ; Zheng, YT
刊名	comparative biochemistry and physiology c-toxicology & pharmacology
	2006
卷号	142 期号:1-2 页码:111-117
关键词	HIV-1 antiviral activity entry inhibitor CVL beta-galactose-specific lectin natural products marine invertebrates Chaetopterus variopedatus
英文摘要	a 30 kda beta-galactose-specific lectin named cvl was isolated from the polychaete marine worm chaetopterus variopedatus (annelida) and its anti-hiv-1 activity in vitro was determined. results showed that cvl inhibited cytopathic effect induced by hiv-1 and the production of viral p24 antigen. the ec50 values were 0.0043 and 0.057 mu m, respectively. time-of-addition analysis of anti-hiv-1 activity indicated its action was at the early stage of virus replication. cvl could blocked the cell-to-cell fusion process of hiv infected and uninfected cells with an ec50 of 0.073 mu m. the inhibition of hiv-1 entry into host cells was demonstrated by using fluorescence-based real-time quantify pcr. at cvl concentration of 0.33 mu m and 0.07 mu m, 86% and 21% virus attachment were blocked, respectively. the anti-hiv-1 action of cvl might relate to blockade of hiv-1 entry into cells. (c) 2005 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology
研究领域[WOS]	biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology
关键词[WOS]	human-immunodeficiency-virus ; envelope glycoprotein gp120 ; binding lectin ; cyanovirin-n ; antiviral activities ; cucumaria-echinata ; plant-lectins ; t-cells ; purification ; mannose
收录类别	SCI
语种	英语
WOS记录号	WOS:000235916600013
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139950]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Zool, Lab Mol Immunopharmacol, Kunming 650223, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Branch, Vladivostok 690022, Russia 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, JH,Jing, K,Wei, L,et al. A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity[J]. comparative biochemistry and physiology c-toxicology & pharmacology,2006,142(1-2):111-117.
APA	Wang, JH.,Jing, K.,Wei, L.,Molchanova, V.,Chikalovets, I.,...&Zheng, YT.(2006).A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity.comparative biochemistry and physiology c-toxicology & pharmacology,142(1-2),111-117.
MLA	Wang, JH,et al."A beta-galactose-specific lectin isolated from the marine worm Chaetopterus variopedatus possesses anti-HIV-1 activity".comparative biochemistry and physiology c-toxicology & pharmacology 142.1-2(2006):111-117.