Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay

	Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay
	Yuan, JL; Wang, GL
刊名	journal of fluorescence
	2005-07-01
卷号	15 期号:4 页码:559-568
关键词	lanthanide complex fluorescence label time-resolved fluorescence bioassay
英文摘要	different from organic fluorescence dyes, fluorescent lanthanide complexes have the fluorescence properties of long fluorescence lifetime, large stokes shift and sharp emission profile, which makes them favorable be used as the fluorescent labeling reagents for microsecond time-resolved fluorescence bioassay. lanthanide complex-based fluorescence labels have been successfully used for highly sensitive time-resolved fluorescence immunoassay, dna hybridization assay, cell activity assay, and bioimaging microscopy assay. since the technique allows easy distinction of the specific fluorescence signal of the long-lived label from short-lived background noises associated with biological samples, scattering lights (tyndall, rayleigh and raman scatterings) and the optical components (cuvettes, filters and lenses), the sensitivity of fluorescence bioassay has been remarkably improved. this paper summarized the recent developments of lanthanide complex-based fluorescence labels and their applications in time-resolved fluorescence bioassays mainly based on the authors' researches and relative publications.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	diketonate-europium chelate ; prostate-specific antigen ; highly sensitive detection ; dna hybridization assay ; state energy-level ; fluorometric applications ; luminescence properties ; environmental-samples ; quantitative measurement ; bioanalytical assays
收录类别	SCI
语种	英语
WOS记录号	WOS:000231940700012
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139765]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Yuan, JL,Wang, GL. Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay[J]. journal of fluorescence,2005,15(4):559-568.
APA	Yuan, JL,&Wang, GL.(2005).Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay.journal of fluorescence,15(4),559-568.
MLA	Yuan, JL,et al."Lanthanide complex-based fluorescence label for time-resolved fluorescence bioassay".journal of fluorescence 15.4(2005):559-568.