Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles

	Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles
	Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL
刊名	journal of fluorescence
	2005-07-01
卷号	15 期号:4 页码:499-505
关键词	terbium complex fluorescent nanoparticle fluorescence probe time-resolved fluoroimmunoassay
英文摘要	novel zirconia-based fluorescent terbium nanoparticles have been prepared as a fluorescent nanoprobe for time-resolved fluorescence bioassay. the nanoparticles were prepared in a water-in-oil (w/o) microemulsion consisting of a strongly fluorescent tb3+ complex, n, n, n-1, n-1-[2,6- bis(3'-aminomethyl-1 - pyrazolyl)- phenylpyridine] tetrakis( acetate)- tb3+(bpta-tb3+), triton x-100, hexanol, and cyclohexane by controlling co-condensation of zr(och2ch3)(4) and zrocl2. the characterizations by transmission electron microscopy and fluorometric methods indicate that the nanoparticles are uniform in size, 33 +/- 4 nm in diameter, and have a fluorescence quantum yield of 8.9% and a long fluorescence lifetime of 2.0 ms. the zirconia-based fluorescent terbium nanoparticles show high stability against basic dissolution in a high ph aqueous buffer compared to the silica-based nanoparticles. a surface modification and bioconjugation method for the fluorescent nanoparticles was developed, and the nanoparticle-conjugated streptavidin (sa) was used for time-resolved floroimmunoassy (tr-fia) of human prostate specific antigen (psa). the result shows that the zirconia-based fluorescent terbium nanoparticles are useful as a fluorescent nanoprobe for time-resolved fluorescence bioassay.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	time-resolved fluoroimmunoassay ; performance liquid-chromatography ; modified porous zirconia ; bioconjugated nanoparticles ; fluorometric application ; europium nanoparticles ; amino-acids ; proteins ; silica ; temperature
收录类别	SCI
语种	英语
WOS记录号	WOS:000231940700006
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139646]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Ye, ZQ,Tan, MQ,Wang, GL,et al. Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles[J]. journal of fluorescence,2005,15(4):499-505.
APA	Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2005).Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles.journal of fluorescence,15(4),499-505.
MLA	Ye, ZQ,et al."Preparation, characterization and application of fluorescent terbium complex-doped zirconia nanoparticles".journal of fluorescence 15.4(2005):499-505.