Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface

	Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface
	Huang, ZX; Ji, DM; Xia, AD
刊名	colloids and surfaces a-physicochemical and engineering aspects
	2005-05-05
卷号	257-58 页码:203-209
关键词	single molecule fluorescence fluctuation reverse intersystem crossing on/off-time dynamic processes
英文摘要	single molecule fluorescence spectroscopy is a powerful tool to reveal the fluorescence fluctuation dynamics of a fluorescence molecule immobilized on a surface. these fluctuations are correlated in time under different excitation intensities with different repetition rate, which may provide the information for us to describe the reasonable excited state processes as well as the surrounding environments. in this report, we showed the first results on the fluorescence fluctuations of a single cy5 molecule at the air-glass interface excited with different conditions by using far-field confocal laser microscopy in conjunction with multichannel scale (mcs) and time-correlated single photon counting (tcspc). we found that the on/off-time and count rates showed different behaviors under different excitation modes. it was found that the excitation with the high repetition rate and/or strong intensity may cause high count rate, and increase the possibility of reverse intersystem crossing from t,, to s. state. which may lead to longer on-time and shorter off-time. furthermore, the photoinduced dynamic processes excited with strong peak power described by a five-level model under continuous wave (cw) and pulsed excitations with high repetition rate, it can be roughly simplified by a three-level scheme under pulse laser at lower repetition rate. (c) 2004 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, physical
研究领域[WOS]	chemistry
关键词[WOS]	room-temperature ; correlation spectroscopy ; photophysical dynamics ; electric dipoles ; quantum jumps ; cyanine dyes ; interface ; microscopy ; excitation
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000228321500039
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139644]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Inst Chem, Ctr Mol Sci, State Key Lab Mol React Dynam, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Huang, ZX,Ji, DM,Xia, AD. Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface[J]. colloids and surfaces a-physicochemical and engineering aspects,2005,257-58:203-209.
APA	Huang, ZX,Ji, DM,&Xia, AD.(2005).Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface.colloids and surfaces a-physicochemical and engineering aspects,257-58,203-209.
MLA	Huang, ZX,et al."Fluorescence intensity and lifetime fluctuations of single Cy5 molecules immobilized on the glass surface".colloids and surfaces a-physicochemical and engineering aspects 257-58(2005):203-209.