Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles | |
Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL | |
刊名 | analytical chemistry
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2004-02-01 | |
卷号 | 76期号:3页码:513-518 |
英文摘要 | novel silica-coated terbium(iii) chelate fluorescent nanoparticles have been prepared and characterized as a new type of fluorescence probe for highly sensitive time-resolved fluorescence bioassay. the preparation was carried out in a water-in-oil microemulsion containing a strongly fluorescent tb3+ chelate, n,n,n-1,n-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-phenylpyridinene]tetrakis(acetate)-tb3+, triton x-100, hexanol, and cyclohexane by controlling hydrolysis of tetraethyl orthosilicate. the nanoparticles are spherical and uniform in size, 42 +/- 3 nm in diameter, strongly fluorescent, and highly photostable and have enough of a long fluorescence lifetime (1.52 ms) for time-resolved fluorescence measurement. a stable and nontoxic method was developed for the surface modification and protein immobilization of the nanoparticles. as a model of application, the nanoparticle-labeled streptavidin was prepared and used in a sandwich-type time-resolved fluoroimmunoassay of human prostate-specific antigen (psa) by using a 96-well microtiter plate as the solid-phase carrier. the method gives a detection limit of 7.0 pg/ml for the psa assay. |
WOS标题词 | science & technology ; physical sciences |
类目[WOS] | chemistry, analytical |
研究领域[WOS] | chemistry |
关键词[WOS] | prostate-specific antigen ; immunofluorometric assay ; ultrasensitive detection ; bioanalytical assays ; reverse micelle ; chelate ; fluoroimmunoassay ; hybridization ; microemulsion ; immunoassays |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000188823900008 |
公开日期 | 2015-11-10 |
内容类型 | 期刊论文 |
源URL | [http://159.226.238.44/handle/321008/139512] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China |
推荐引用方式 GB/T 7714 | Ye, ZQ,Tan, MQ,Wang, GL,et al. Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles[J]. analytical chemistry,2004,76(3):513-518. |
APA | Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2004).Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles.analytical chemistry,76(3),513-518. |
MLA | Ye, ZQ,et al."Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles".analytical chemistry 76.3(2004):513-518. |
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