Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles

	Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles
	Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL
刊名	analytical chemistry
	2004-02-01
卷号	76 期号:3 页码:513-518
英文摘要	novel silica-coated terbium(iii) chelate fluorescent nanoparticles have been prepared and characterized as a new type of fluorescence probe for highly sensitive time-resolved fluorescence bioassay. the preparation was carried out in a water-in-oil microemulsion containing a strongly fluorescent tb3+ chelate, n,n,n-1,n-1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-phenylpyridinene]tetrakis(acetate)-tb3+, triton x-100, hexanol, and cyclohexane by controlling hydrolysis of tetraethyl orthosilicate. the nanoparticles are spherical and uniform in size, 42 +/- 3 nm in diameter, strongly fluorescent, and highly photostable and have enough of a long fluorescence lifetime (1.52 ms) for time-resolved fluorescence measurement. a stable and nontoxic method was developed for the surface modification and protein immobilization of the nanoparticles. as a model of application, the nanoparticle-labeled streptavidin was prepared and used in a sandwich-type time-resolved fluoroimmunoassay of human prostate-specific antigen (psa) by using a 96-well microtiter plate as the solid-phase carrier. the method gives a detection limit of 7.0 pg/ml for the psa assay.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	prostate-specific antigen ; immunofluorometric assay ; ultrasensitive detection ; bioanalytical assays ; reverse micelle ; chelate ; fluoroimmunoassay ; hybridization ; microemulsion ; immunoassays
收录类别	SCI
语种	英语
WOS记录号	WOS:000188823900008
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139512]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China
推荐引用方式 GB/T 7714	Ye, ZQ,Tan, MQ,Wang, GL,et al. Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles[J]. analytical chemistry,2004,76(3):513-518.
APA	Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2004).Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles.analytical chemistry,76(3),513-518.
MLA	Ye, ZQ,et al."Preparation, characterization, and time-resolved fluorometric application of silica-coated terbium(ill) fluorescent nanoparticles".analytical chemistry 76.3(2004):513-518.