THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION

	THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION
	Chai, Xi-Jun; Lin, Yi-Zheng; Qin, Jian-Jun; Jiang, Cheng-Zhang
刊名	biotechnology techniques
	1992-07-01
卷号	6 期号:4 页码:381-384
英文摘要	recombinant hepatitis b surface antigen (hbsag) broth was purified by diafiltration with a hollow fibre ultrafiltration membrane (cut off 100kda), by pre-treating the hbsag broth with enzyme, most of the contaminating proteins was removed and very high recovery ratio of hbsag was achieved. when hbsag solution was concentrated using hollow fibre ultrafiltration membrane to 10% of its original volume, the hbsag was recovered almost completely. accordingly, membrane purification of hbsag is a high yield, fast method.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biotechnology & applied microbiology
研究领域[WOS]	biochemistry & molecular biology ; biotechnology & applied microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000207027400018
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139410]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dept Membrane Proc, Dalian 116012, Peoples R China
推荐引用方式 GB/T 7714	Chai, Xi-Jun,Lin, Yi-Zheng,Qin, Jian-Jun,et al. THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION[J]. biotechnology techniques,1992,6(4):381-384.
APA	Chai, Xi-Jun,Lin, Yi-Zheng,Qin, Jian-Jun,&Jiang, Cheng-Zhang.(1992).THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION.biotechnology techniques,6(4),381-384.
MLA	Chai, Xi-Jun,et al."THE PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN BY HOLLOW FIBRE ULTRAFILTRATION MEMBRANE DIAFILTRATION".biotechnology techniques 6.4(1992):381-384.