Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion

	Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion
	Mao, XL ; Wang, K ; Du, YG ; Lin, BC
刊名	electrophoresis
	2003-09-01
卷号	24 期号:18 页码:3273-3278
关键词	chicken ovalbumin glycopeptides microchip electrophoresis miniaturization peptide mapping Turkey ovalburnin
英文摘要	the polypeptide and carbohydrate patterns of two glycoproteins, chicken ovalbumin (co) and turkey ovalbumin (to), were analyzed by microchip electrophoresis (me), following digestion with proteases and exoglycosidases. glycopeptides derived from ovalbumin were obtained by digestion with pronase, followed by dialysis, and then separated by me. using co as model, the method was developed to deduce the structure of glycans from glycoproteins by comparing the electropherograms of glycopeptides with and without digestion of exolycosidases. applying the same approach, the structure of oligosaccharides linked to to was determined. to was found to contain high-mannose type oligosaccharides and oligosaccharides with terminal n-acetylglucosamine residues. the complete primary analysis of co and to by me described in this paper provides a basis for an analysis of glycoproteins with an integrated microfluidic chip.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	capillary-electrophoresis ; mass-spectrometry ; ovalbumin ; glycosylation ; glycoproteins ; oligosaccharides ; proteomics ; sequence ; system ; chip
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000185897100026
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139241]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Shanghai Acad Life Sci, Inst Biochem & Cell Biol, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Mao, XL,Wang, K,Du, YG,et al. Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion[J]. electrophoresis,2003,24(18):3273-3278.
APA	Mao, XL,Wang, K,Du, YG,&Lin, BC.(2003).Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion.electrophoresis,24(18),3273-3278.
MLA	Mao, XL,et al."Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion".electrophoresis 24.18(2003):3273-3278.