Chiral separation by capillary electrochromatography with physically adsorbed stationary phase and dynamically modified stationary phase

	Chiral separation by capillary electrochromatography with physically adsorbed stationary phase and dynamically modified stationary phase
	Ye, ML; Zou, HF; Lei, ZD; Wu, RN; Li, JY
刊名	chinese journal of analytical chemistry
	2001-03-01
卷号	29 期号:3 页码:299-302
关键词	capillary electrochromatography dynamical modification adsorbed stationary phase enantiomer separation
英文摘要	a novel mode of chiral separation in electrochromatogaphy (cec) with dynamically modified stationary phase (dms-cec) was presented. the capillary column was packed with strong anionic exchange stationary phase, the sulfated beta -cyclodextrin (s-cd), which was added in the mobile phase, dynamically adsorbed to the packing surface and a new layer of chiral stationary phase was formed. the separation of enantiomer was based on their different interaction with the new stationary phase. the enantionmers of tryptophan, atropine and verapamil were successfully separated in this system with resolution of 2.06, 10.1 and 1.96, and the column efficiency for the enantiomers were varied from 85000 plates/m to 412000 plates/m. the relative standard deviation (rsd) of void time and the tryptophan enantiomers' migration time for 17 consecutive runs were 0.5%, 0.6% and 0.7%, respectively. enantiomer separation by capillary electrochromatogaphy with absorbed bovine serum albumin (bsa) and sulfated cyclodextrin (s-cd) as chiral stationary phases were also studied. the resolution for tryptophan enantiomers in the two systems were 3.86 and 2.97, respectively. it was found that the superiority of dms-cec over the adsorbed s-cd column cec was that better repeatability could be obtained in dris-cec.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	modified cationic surfactant ; beta-cyclodextrin ; enantiomers ; silica ; column
收录类别	SCI
语种	英语
WOS记录号	WOS:000167761200014
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/139040]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Ye, ML,Zou, HF,Lei, ZD,et al. Chiral separation by capillary electrochromatography with physically adsorbed stationary phase and dynamically modified stationary phase[J]. chinese journal of analytical chemistry,2001,29(3):299-302.
APA	Ye, ML,Zou, HF,Lei, ZD,Wu, RN,&Li, JY.(2001).Chiral separation by capillary electrochromatography with physically adsorbed stationary phase and dynamically modified stationary phase.chinese journal of analytical chemistry,29(3),299-302.
MLA	Ye, ML,et al."Chiral separation by capillary electrochromatography with physically adsorbed stationary phase and dynamically modified stationary phase".chinese journal of analytical chemistry 29.3(2001):299-302.