Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography

	Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography
	Luo, QZ; Zou, HF; Wang, HL; Mao, XQ; Kong, L; Ni, JY
刊名	chinese journal of analytical chemistry
	2001-05-01
卷号	29 期号:5 页码:497-501
关键词	affinity chromatography polymer monolithic protein A human immunoglobulin G
英文摘要	reactive continuous rods of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) have been prepared by "in-situ" copolymerization of the monomers in the presence of porogenic dilute, and protein a was immobilized on the continuous rods directly or through a 11 carbon atom space arm with molded synthetic methods. the properties of these two rods columns were characterized and the results showed that the medium with space arm had some extent hydrophobicity. the affinity column without space arm was used to determine the human immunoglobulin g (higg) in human serum. the correlative coefficient of the calibration curve was 0.999; the effect of the flow rate on the pressure of the continuous rods was also investigated. the subclasses of higg were separated by the use of a ph gradient.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	separation ; membrane
收录类别	SCI
语种	英语
WOS记录号	WOS:000168992300001
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138929]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Luo, QZ,Zou, HF,Wang, HL,et al. Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography[J]. chinese journal of analytical chemistry,2001,29(5):497-501.
APA	Luo, QZ,Zou, HF,Wang, HL,Mao, XQ,Kong, L,&Ni, JY.(2001).Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography.chinese journal of analytical chemistry,29(5),497-501.
MLA	Luo, QZ,et al."Preparation and characterization of protein A immobilized on molded monolithic rod of macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) for affinity chromatography".chinese journal of analytical chemistry 29.5(2001):497-501.