Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption

	Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption
	Gan, HY; Shang, ZH; Wang, JD; Liu, XL
刊名	chromatographia
	2001-04-01
卷号	53 期号:7-8 页码:450-453
关键词	affinity nylon membranes pore-size effects hydroxyethylcellulose gamma-globulin adsorption human plasma
英文摘要	l-phenylalanine was immobilized on nylon membranes with two pore diameters (0.45 mum and 1 mum), by activation with 1,4-butanediol diglycidyl ether, and the effect of pore size on the affinity adsorption of gamma -globulin studied by batch and kinetic methods. experiment shows that adsorption on both affinity membranes obeys the freundlich model. the accessible pore volume for adsorption of proteins on the membrane with 0.45 mum diameter pores is less than for that with 1 mum diameter pores. the adsorption capacity of affinity membranes with 1 mum diameter pores is 2.5-fold that of membranes with 0.45 mum diameter pores. feed-rate has a larger effect on affinity adsorption on the membrane with 0.45 mum diameter pores than on that with 1 mum diameter pores. small pores on the affinity membrane do not cause broadening of the elution peak. it is concluded that affinity interaction and separation occur mainly in the large pores, and small pore size does not favor improvement of adsorption capacity, gamma -globulin 85.1% pure can be obtained in one step from human plasma by use of the affinity membrane with 0.45 mum diameter pores.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	chromatography ; performance ; proteins
收录类别	SCI
语种	英语
WOS记录号	WOS:000168406700018
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138852]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Gan, HY,Shang, ZH,Wang, JD,et al. Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption[J]. chromatographia,2001,53(7-8):450-453.
APA	Gan, HY,Shang, ZH,Wang, JD,&Liu, XL.(2001).Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption.chromatographia,53(7-8),450-453.
MLA	Gan, HY,et al."Effect of the pore sizes of nylon affinity membranes on gamma-globulin adsorption".chromatographia 53.7-8(2001):450-453.