Highly efficient separation of proteins using high performance ion exchange membrane chromatography

	Highly efficient separation of proteins using high performance ion exchange membrane chromatography
	Zhou, DM; Zou, HF; Yang, L; Ni, JY; Wang, HL; Zhang, YK
刊名	chinese journal of analytical chemistry
	1999-12-01
卷号	27 期号:12 页码:1370-1375
关键词	cellulose membrane ion-exchange proteins
英文摘要	an anion exchange membrane medium with diethylammonium group was prepared. it has been used for the separation of three standard proteins by utilization of new membrane cartridge. this new cartridge could solve the two technical problems of the leakage and non-homogeneous distribution of the sample, which had hampered the further use of hight performance membrane chromatography. the separation was optimized. compared with the conventional membrane cartridge, this new cartridge has higher column efficiency, and lower column pressure than that on the conventional hplc column, so that the high-pressure system is not needed for this cartridge. the separation could be carried out at the flow rate of 3.0 ml/min and one separation takes only 2 min. the separations under different elution gradients and different flow rates were compared. for the tested proteins, the increase of flow rate could not only shorten the separation time but also improve the separation.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	purification
收录类别	SCI
语种	英语
WOS记录号	WOS:000084530900002
公开日期	2015-11-10
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/138600]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
推荐引用方式 GB/T 7714	Zhou, DM,Zou, HF,Yang, L,et al. Highly efficient separation of proteins using high performance ion exchange membrane chromatography[J]. chinese journal of analytical chemistry,1999,27(12):1370-1375.
APA	Zhou, DM,Zou, HF,Yang, L,Ni, JY,Wang, HL,&Zhang, YK.(1999).Highly efficient separation of proteins using high performance ion exchange membrane chromatography.chinese journal of analytical chemistry,27(12),1370-1375.
MLA	Zhou, DM,et al."Highly efficient separation of proteins using high performance ion exchange membrane chromatography".chinese journal of analytical chemistry 27.12(1999):1370-1375.